ArticleAngewandte Chemie (International ed. in English)2013
Bioorthogonal approach to identify unsuspected drug targets in live cells.
Article in Angewandte Chemie (International ed. in English), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- Proteome-Wide Profiling of Olaparib Interactors Using a Biotinylated Photoaffinity Probe.Chembiochem : a European journal of chemical biology · 2025Article
- Investigating Antiprotozoal Chemotherapies with Novel Proteomic Tools-Chances and Limitations: A Critical Review.International journal of molecular sciences · 2024Review
- Article
- Thiomethyltetrazines Are Reversible Covalent Cysteine Warheads Whose Dynamic Behavior can be "Switched Off"Journal of the American Chemical Society · 2023Article
- Nanoplatform for the Delivery of Topotecan in the Cancer Milieu: An Appraisal of its Therapeutic Efficacy.Cancers · 2022Review
- Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes.Nature biotechnology · 2022Article
- Olaparib-Based Photoaffinity Probes for PARP-1 Detection in Living Cells.Chembiochem : a European journal of chemical biology · 2020Article
- Ultra-fast cycling for multiplexed cellular fluorescence imaging.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2020Article
- Ultra-fast Cycling for Multiplexed Cellular Fluorescence Imaging.Angewandte Chemie (International ed. in English) · 2020Article
- Tryptophan scanning mutagenesis as a way to mimic the compound-bound state and probe the selectivity of allosteric inhibitors in cells.Chemical science · 2020Article
- Biomedical applications of copper-free click chemistry:Chemical science · 2019Review
- Article
- Targeting strategies for improving the efficacy of nanomedicine in oncology.Beilstein journal of nanotechnology · 2019Review
- Constructing New Bioorthogonal Reagents and Reactions.Accounts of chemical research · 2018Article
- Developments of bioorthogonal handle-containing photo-crosslinkers for photoaffinity labeling.MedChemComm · 2017Review
- Measurement of drug-target engagement in live cells by two-photon fluorescence anisotropy imaging.Nature protocols · 2017Article
- Development of inverse electron demand Diels-Alder ligation and TR-FRET assays for the determination of ligand-protein target occupancy in live cells.MedChemComm · 2017Article
- Proteome-wide Profiling of Clinical PARP Inhibitors Reveals Compound-Specific Secondary Targets.Cell chemical biology · 2016Article
- Click Chemistry-Mediated Nanosensors for Biochemical Assays.Theranostics · 2016Review
- Tetrazine ligation for chemical proteomics.Proteome science · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A proteomics method to pull down secondary drug targets from live cells is described. The drug of interest is modified with trans-cyclooctene (TCO) and incubated with live cells. Upon cell lysis, the modified drug bound to the protein is pulled down using magnetic beads decorated with a cleavable tetrazine-modified linker. Samples are then run on an SDS-PAGE gel and isolated bands are submitted for mass spectrometry analysis to identify drug targets.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.