ArticleProceedings of the National Academy of Sciences of the United States of America2013
Role of the ectonucleotidase NTPDase2 in taste bud function.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 98 citations in OpenAlex.
- An efficient functional screening method for anti ATP hydrolase antibody based on fluorescent probes.Frontiers in immunology · 2026Article
- Pig Taste Cell-derived Organoids Synthesize Insulin.Endocrinology · 2025Article
- Bitter and Sweet Diets Alter Taste Response and Alcohol Consumption Behavior in Mice.Nutrients · 2025Article
- Epitope Tagging with Genome Editing in Mice Reveals That the Proton Channel OTOP1 Is Apically Localized and Not Restricted to Type III "Sour" Taste Receptor Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Cerebrospinal Fluid Proteomic Changes after Nusinersen in Patients with Spinal Muscular Atrophy.Journal of clinical medicine · 2023Article
- Article
- Rapid structural remodeling of peripheral taste neurons is independent of taste cell turnover.PLoS biology · 2023Article
- Interleukin (IL)-1 Receptor Signaling Is Required for Complete Taste Bud Regeneration and the Recovery of Neural Taste Responses following Axotomy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Physiology of the tongue with emphasis on taste transduction.Physiological reviews · 2023Review
- Article
- Purinergic Signaling in Oral Tissues.International journal of molecular sciences · 2022Review
- METTL3-mediated mInternational journal of oral science · 2022Article
- Morphology and chemical characteristics of taste buds associated with P2X3-immunoreactive afferent nerve endings in the rat incisive papilla.Journal of anatomy · 2022Article
- The Role of ATP and Purinergic Receptors in Taste Signaling.Handbook of experimental pharmacology · 2022Review
- "Tripartite Synapses" in Taste Buds: A Role for Type I Glial-like Taste Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Purinergic signaling as a new mechanism underlying physical exercise benefits: a narrative review.Purinergic signalling · 2021Review
- Purinergic neurotransmission in the gustatory system.Autonomic neuroscience : basic & clinical · 2021Article
- Functional expression of TMEM16A in taste bud cells.The Journal of physiology · 2021Article
- Ectonucleotidases in Inflammation, Immunity, and Cancer.Journal of immunology (Baltimore, Md. : 1950) · 2021Review
- Ectonucleoside triphosphate diphosphohydrolases and ecto-5'-nucleotidase in purinergic signaling: how the field developed and where we are now.Purinergic signalling · 2021Review
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Taste buds are unusual in requiring ATP as a transmitter to activate sensory nerve fibers. In response to taste stimuli, taste cells release ATP, activating purinergic receptors containing the P2X2 and P2X3 subunits on taste nerves. In turn, the released ATP is hydrolyzed to ADP by a plasma membrane nucleoside triphosphate previously identified as nucleoside triphosphate diphosphohydrolase-2 (NTPDase2). In this paper we investigate the role of this ectonucleotidase in the function of taste buds by examining gene-targeted Entpd2-null mice globally lacking NTPDase2. RT-PCR confirmed the absence of NTPDase2, and ATPase enzyme histochemistry reveals no reaction product in taste buds of knockout mice, suggesting that NTPDase2 is the dominant form in taste buds. RT-PCR and immunocytochemistry demonstrated that in knockout mice all cell types are present in taste buds, even those cells normally expressing NTPDase2. In addition, the overall number and size of taste buds are normal in Entpd2-null mice. Luciferin/luciferase assays of circumvallate tissue of knockout mice detected elevated levels of extracellular ATP. Electrophysiological recordings from two taste nerves, the chorda tympani and glossopharyngeal, revealed depressed responses to all taste stimuli in Entpd2-null mice. Responses were more depressed in the glossopharyngeal nerve than in the chorda tympani nerve and involved all taste qualities; responses in the chorda tympani were more depressed to sweet and umami stimuli than to other qualities. We suggest that the excessive levels of extracellular ATP in the Entpd2-knockout animals desensitize the P2X receptors associated with nerve fibers, thereby depressing taste responses.
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