ArticleProceedings of the National Academy of Sciences of the United States of America2013
Gene duplication in the major insecticide target site, Rdl, in Drosophila melanogaster.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 79 citations in OpenAlex.
- From bench to farm: leveragingFly · 2027Review
- Metabolic resistance of the tiger mosquito to pyrethroid insecticides in La Réunion Island likely results from local adaptation.Genome biology and evolution · 2026Article
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- Despite structural identity, ace-1 heterogenous duplication resistance alleles are quite diverse in Anopheles mosquitoes.Heredity · 2024Article
- Transposon accumulation at xenobiotic gene family loci in aphids.Genome research · 2023Article
- Transposable elements and xenobiotic resistance.Frontiers in insect science · 2023Review
- A next generation targeted amplicon sequencing method to screen for insecticide resistance mutations in Aedes aegypti populations reveals a rdl mutation in mosquitoes from Cabo Verde.PLoS neglected tropical diseases · 2022Article
- Dominance shifts increase the likelihood of soft selective sweeps.Evolution; international journal of organic evolution · 2022Article
- Fitness effects for Ace insecticide resistance mutations are determined by ambient temperature.BMC biology · 2020Article
- The genome of pest Rhynchophorus ferrugineus reveals gene families important at the plant-beetle interface.Communications biology · 2020Article
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- Whole-genome sequencing reveals high complexity of copy number variation at insecticide resistance loci in malaria mosquitoes.Genome research · 2019Article
- A likelihood ratio test for changes in homeolog expression bias.BMC bioinformatics · 2019Article
- Small Segmental Duplications in Drosophila-High Rate of Emergence and Elimination.Genome biology and evolution · 2019Article
- Adaptive deletion in resistance gene duplications in the malaria vectorEvolutionary applications · 2018Article
- Copy number variation (CNV) and insecticide resistance in mosquitoes: evolving knowledge or an evolving problem?Current opinion in insect science · 2018Review
- Candidate-gene based GWAS identifies reproducible DNA markers for metabolic pyrethroid resistance from standing genetic variation in East African Anopheles gambiae.Scientific reports · 2018Article
- Influence of the RDL A301S mutation in the brown planthopper Nilaparvata lugens on the activity of phenylpyrazole insecticides.Pesticide biochemistry and physiology · 2017Article
- Local selection in the presence of high levels of gene flow: Evidence of heterogeneous insecticide selection pressure across Ugandan Culex quinquefasciatus populations.PLoS neglected tropical diseases · 2017Article
- Heterogeneous gene duplications can be adaptive because they permanently associate overdominant alleles.Evolution letters · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Resistance to Dieldrin gene, Rdl, encodes a GABA-gated chloride channel subunit that is targeted by cyclodiene and phenylpyrazole insecticides. The gene was first characterized in Drosophila melanogaster by genetic mapping of resistance to the cyclodiene dieldrin. The 4,000-fold resistance observed was due to a single amino acid replacement, Ala(301) to Ser. The equivalent change was subsequently identified in Rdl orthologs of a large range of resistant insect species. Here, we report identification of a duplication at the Rdl locus in D. melanogaster. The 113-kb duplication contains one WT copy of Rdl and a second copy with two point mutations: an Ala(301) to Ser resistance mutation and Met(360) to Ile replacement. Individuals with this duplication exhibit intermediate dieldrin resistance compared with single copy Ser(301) homozygotes, reduced temperature sensitivity, and altered RNA editing associated with the resistant allele. Ectopic recombination between Roo transposable elements is involved in generating this genomic rearrangement. The duplication phenotypes were confirmed by construction of a transgenic, artificial duplication integrating the 55.7-kb Rdl locus with a Ser(301) change into an Ala(301) background. Gene duplications can contribute significantly to the evolution of insecticide resistance, most commonly by increasing the amount of gene product produced. Here however, duplication of the Rdl target site creates permanent heterozygosity, providing unique potential for adaptive mutations to accrue in one copy, without abolishing the endogenous role of an essential gene.
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