ArticleStem cell research2013
Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence.
Article in Stem cell research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 61 citations in OpenAlex.
- Is All Fat Created Equal? A Comparative Study of Chondrogenesis Potential of Peri-Ovarian Adipose Tissues in Dogs.Animals : an open access journal from MDPI · 2026Article
- Bone marrow stromal cell-derived hepcidin has antimicrobial and immunomodulatory activities.Scientific reports · 2024Article
- Deconstructing heterogeneity of replicative senescence in human mesenchymal stem cells at single cell resolution.GeroScience · 2024Article
- The Potential Use of THP-1, a Monocytic Leukemia Cell Line, to Predict Immune-Suppressive Potency of Human Bone-Marrow Stromal Cells (BMSCs) In Vitro: A Pilot Study.International journal of molecular sciences · 2023Article
- Article
- Are the Properties of Bone Marrow-Derived Mesenchymal Stem Cells Influenced by Overweight and Obesity?International journal of molecular sciences · 2023Review
- CD26 is a senescence marker associated with reduced immunopotency of human adipose tissue-derived multipotent mesenchymal stromal cells.Stem cell research & therapy · 2022Article
- Article
- Regulation of pathophysiological and tissue regenerative functions of MSCs mediated via the WNT signaling pathway (Review).Molecular medicine reports · 2021Review
- Transcriptomic alterations underline aging of osteogenic bone marrow stromal cells.World journal of stem cells · 2021Article
- To Breathe or Not to Breathe: The Role of Oxygen in Bone Marrow-Derived Mesenchymal Stromal Cell Senescence.Stem cells international · 2021Review
- Accumulating Transcriptome Drift Precedes Cell Aging in Human Umbilical Cord-Derived Mesenchymal Stromal Cells Serially Cultured to Replicative Senescence.Stem cells translational medicine · 2019Article
- Building Strong Neighborhoods in the Lung with a Little Help from My Mesenchymal Stem Cells.American journal of respiratory and critical care medicine · 2019Article
- In vitro expansion affects the response of human bone marrow stromal cells to irradiation.Stem cell research & therapy · 2019Article
- Mesenchymal stromal cells from infants with simple polydactyly modulate immune responses more efficiently than adult mesenchymal stromal cells.Cytotherapy · 2019Article
- Single cell sequencing reveals gene expression signatures associated with bone marrow stromal cell subpopulations and time in culture.Journal of translational medicine · 2019Article
- Molecular Profiles of Cell-to-Cell Variation in the Regenerative Potential of Mesenchymal Stromal Cells.Stem cells international · 2019Review
- Comparison of human bone marrow stromal cells cultured in human platelet growth factors and fetal bovine serum.Journal of translational medicine · 2018Article
- Identification of new genes associated to senescent and tumorigenic phenotypes in mesenchymal stem cells.Scientific reports · 2017Article
- Isolation and prolonged expansion of oral mesenchymal stem cells under clinical-grade, GMP-compliant conditions differentially affects "stemness" properties.Stem cell research & therapy · 2017Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
The outcomes of clinical trials using bone marrow stromal cell (BMSC) are variable; the degree of the expansion of BMSCs during clinical manufacturing may contribute to this variability since cell expansion is limited by senescence. Human BMSCs from aspirates of healthy subjects were subcultured serially until cell growth stopped. Phenotype and functional measurements of BMSCs from two subjects including senescence-associated beta-galactosidase staining and colony formation efficiency changed from an early to a senescence pattern at passage 6 or 7. Transcriptome analysis of 10 early and 15 late passage BMSC samples from 5 subjects revealed 2122 differentially expressed genes, which were associated with immune response, development, and cell proliferation pathways. Analysis of 57 serial BMSC samples from 7 donors revealed that the change from an early to senescent profile was variable among subjects and occurred prior to changes in phenotypes. BMSC age expressed as a percentage of maximum population doublings (PDs) was a good indicator for an early or senescence transcription signature but this measure of BMSC life span can only be calculated after expanding BMSCs to senescence. In order to find a more useful surrogate measure of BMSC age, we used a computational biology approach to identify a set of genes whose expression at each passage would predict elapsed age of BMSCs. A total of 155 genes were highly correlated with BMSC age. A least angle regression algorithm identified a set of 24 BMSC age-predictive genes. In conclusion, the onset of senescence-associated molecular changes was variable and preceded changes in other indicators of BMSC quality and senescence. The 24 BMSC age predictive genes will be useful in assessing the quality of clinical BMSC products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.