ArticlePlant physiology2013
The green microalga Chlamydomonas reinhardtii has a single ω-3 fatty acid desaturase that localizes to the chloroplast and impacts both plastidic and extraplastidic membrane lipids.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 98 citations in OpenAlex.
- Mechanisms of cold adaptation in a novel marine eukaryotic phytoplankton-psychrophilic microalga Chlamydomonas sp. LWD-1.Plant cell reports · 2026Article
- Knocking out the carboxyltransferase interactor 1 (CTI1) in Chlamydomonas boosted oil content by fivefold without affecting cell growth.Plant biotechnology journal · 2025Article
- Phosphorus starvation induces the synthesis of novel lipid class diacylglyceryl glucuronide and diacylglyceryl-N,N,N-trimethylhomoserine in two species of cold-adapted microalgae Raphidonema (Chlorophyta).The Plant journal : for cell and molecular biology · 2025Article
- Molecular cloning and functional analysis of a plastidial ω3 desaturase fromFrontiers in microbiology · 2024Article
- Towards microalga-based superfoods: heterologous expression of zeolin inFrontiers in plant science · 2023Article
- Phenotype stability and dynamics of transposable elements in a strain of the microalga Tisochrysis lutea with improved lipid traits.PloS one · 2023Article
- Enhanced accumulation of oil through co-expression of fatty acid and ABC transporters in Chlamydomonas under standard growth conditions.Biotechnology for biofuels and bioproducts · 2022Article
- Key Enzymes in Fatty Acid Synthesis Pathway for Bioactive Lipids Biosynthesis.Frontiers in nutrition · 2022Review
- Characterization of fatty acid desaturases reveals stress-induced synthesis of C18 unsaturated fatty acids enriched in triacylglycerol in the oleaginous alga Chromochloris zofingiensis.Biotechnology for biofuels · 2021Article
- The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis.Biotechnology for biofuels · 2021Review
- A multi-omic characterization of temperature stress in a halotolerant Scenedesmus strain for algal biotechnology.Communications biology · 2021Article
- Temperature Acclimation of the PicoalgaFrontiers in plant science · 2021Article
- The Oleic/Linoleic Acid Ratio in Olive (Frontiers in plant science · 2021Article
- Plastidic Δ6 Fatty-Acid Desaturases with Distinctive Substrate Specificity Regulate the Pool of C18-PUFAs in the Ancestral PicoalgaPlant physiology · 2020Article
- Fatty acid synthesis by Chlamydomonas reinhardtii in phosphorus limitation.Journal of bioenergetics and biomembranes · 2020Article
- CrABCA2 Facilitates Triacylglycerol Accumulation inMolecules and cells · 2020Article
- Influence of nitrogen availability on biomass, lipid production, fatty acid profile, and the expression of fatty acid desaturase genes in Messastrum gracile SE-MC4.World journal of microbiology & biotechnology · 2020Article
- Microalgae with Immunomodulatory Activities.Marine drugs · 2019Review
- Chlorella vulgaris genome assembly and annotation reveals the molecular basis for metabolic acclimation to high light conditions.The Plant journal : for cell and molecular biology · 2019Article
- Phosphorus-Induced Lipid Class Alteration Revealed by Lipidomic and Transcriptomic Profiling in Oleaginous MicroalgaMarine drugs · 2019Article
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9 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The ω-3 polyunsaturated fatty acids account for more than 50% of total fatty acids in the green microalga Chlamydomonas reinhardtii, where they are present in both plastidic and extraplastidic membranes. In an effort to elucidate the lipid desaturation pathways in this model alga, a mutant with more than 65% reduction in total ω-3 fatty acids was isolated by screening an insertional mutant library using gas chromatography-based analysis of total fatty acids of cell pellets. Molecular genetics analyses revealed the insertion of a TOC1 transposon 113 bp upstream of the ATG start codon of a putative ω-3 desaturase (CrFAD7; locus Cre01.g038600). Nuclear genetic complementation of crfad7 using genomic DNA containing CrFAD7 restored the wild-type fatty acid profile. Under standard growth conditions, the mutant is indistinguishable from the wild type except for the fatty acid difference, but when exposed to short-term heat stress, its photosynthesis activity is more thermotolerant than the wild type. A comparative lipidomic analysis of the crfad7 mutant and the wild type revealed reductions in all ω-3 fatty acid-containing plastidic and extraplastidic glycerolipid molecular species. CrFAD7 was localized to the plastid by immunofluorescence in situ hybridization. Transformation of the crfad7 plastidial genome with a codon-optimized CrFAD7 restored the ω-3 fatty acid content of both plastidic and extraplastidic lipids. These results show that CrFAD7 is the only ω-3 fatty acid desaturase expressed in C. reinhardtii, and we discuss possible mechanisms of how a plastid-located desaturase may impact the ω-3 fatty acid content of extraplastidic lipids.
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