ArticleBioMed research international2013
Does cryopreservation of ovarian tissue affect the distribution and function of germinal vesicle oocytes mitochondria?
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 28 citations in OpenAlex.
- Umbilical cord mesenchymal stem cells and their extracellular vesicles attenuate cryopreservation-induced ovarian injury via the suppression of ferroptosis in an in vitro culture system.Materials today. Bio · 2026Article
- Resveratrol enhanced mitochondrial recovery from cryopreservation-induced damages in oocytes and embryos.Reproductive medicine and biology · 2021Review
- Mitochondrial Dysfunction and Oxidative Stress Caused by Cryopreservation in Reproductive Cells.Antioxidants (Basel, Switzerland) · 2021Review
- Initial response of ovarian tissue transcriptome to vitrification or microwave-assisted dehydration in the domestic cat model.BMC genomics · 2020Article
- Assessment of DNA Repair Gene Expressions in Vitrified Mouse Preantral Follicles.Cell journal · 2020Article
- Reactive oxygen species level, mitochondrial transcription factor A gene expression and succinate dehydrogenase activity in metaphase II oocytes derived fromVeterinary research forum : an international quarterly journal · 2018Article
- Calcium ion regulation by BAPTA-AM and ruthenium red improved the fertilisation capacity and developmental ability of vitrified bovine oocytes.Scientific reports · 2017Article
- Effect of Farnesyltransferase Inhibitor R115777 on Mitochondria of Plasmodium falciparum.The Korean journal of parasitology · 2015Article
- Oocyte mitochondrial function and reproduction.Current opinion in obstetrics & gynecology · 2015Review
- Mitochondrial Distribution and ATP Content of Vitrified, In vitro Matured Mouse Oocytes.Avicenna journal of medical biotechnology · 2014Article
- Significance of Mitochondrial Dynamics in Reproductive Physiology: Current and Emerging Horizons in Mitochondrial Therapy for Assisted Reproductive Technologies.Reproductive medicine and biologyReview
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to evaluate mitochondrial alteration and ATP content of germinal vesicle (GV) oocytes isolated from fresh and vitrified ovaries. After superovulation, the ovaries from adult mice were collected and divided into control and vitrified groups. GV oocytes were isolated mechanically from each group. Half were cultured for 24 hours and their maturation was assessed. Metaphase II oocytes were collected and submitted to in vitro fertilization and their fertilization rates and development to the blastocyst stage were evaluated. In the remaining GV oocytes, ATP levels were quantified, and mitochondrial distribution, mitochondrial membrane potential, and intracellular free calcium were detected with rhodamine 123, JC-1 and Flou-4 AM staining, using laser-scanning confocal microscopy. Maturation and fertilization rates of GV oocytes and the developmental rates of subsequent embryos were significantly lower in vitrified samples (P < 0.05). The ATP content and Ca(2+) levels differed significantly in fresh and vitrified GV oocytes (P < 0.05). Most mitochondria were seen as large and homogenous aggregates (66.6%) in fresh GV oocytes compared to vitrified oocytes (50%). No significant differences in mitochondrial membrane potential were found between the groups. The lower maturation and fertilization rates of GV oocytes from vitrified ovaries may be due to changes in their mitochondrial function and distribution.
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