ArticleApplied and environmental microbiology2013
Exoproteome profiles of Clostridium cellulovorans grown on various carbon sources.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- Improvement of arabinoxylan degradation in Clostridium saccharobutylicum DSM 13864Applied microbiology and biotechnology · 2025Article
- Proteomic Characterization of theInternational journal of molecular sciences · 2025Article
- Structural and functional insights into cellulosomes: masters of plant cell wall degradation.Frontiers in microbiology · 2025Review
- Extracytoplasmic polysaccharides control cellulosomal and non-cellulosomal systems in Herbivorax saccincola A7.Applied microbiology and biotechnology · 2024Article
- Enzymatic Characterization of Unused Biomass Degradation Using theMicroorganisms · 2022Article
- Temporal proteome dynamics of Clostridium cellulovorans cultured with major plant cell wall polysaccharides.BMC microbiology · 2019Article
- Biomethane production from sugar beet pulp under cocultivation with Clostridium cellulovorans and methanogens.AMB Express · 2019Article
- Article
- Xylanase B from Clostridium cellulovorans 743B: overexpression, purification, crystallization and X-ray diffraction analysis.Acta crystallographica. Section F, Structural biology communications · 2018Article
- Characteristic strategy of assimilation of various saccharides by Clostridium cellulovorans.AMB Express · 2016Article
- Synergistic properties of cellulases from Clostridium cellulovorans in the presence of cellobiose.AMB Express · 2016Article
- Putative Alginate Assimilation Process of the Marine Bacterium Saccharophagus degradans 2-40 Based on Quantitative Proteomic Analysis.Marine biotechnology (New York, N.Y.) · 2016Article
- Exoproteome analysis ofAMB Express · 2015Article
- Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis.AMB Express · 2015Article
- Direct glucose production from lignocellulose using Clostridium thermocellum cultures supplemented with a thermostable β-glucosidase.Biotechnology for biofuels · 2013Article
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6 authors.
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Abstract
The cellulosome is a complex of cellulosomal proteins bound to scaffolding proteins. This complex is considered the most efficient system for cellulose degradation. Clostridium cellulovorans, which is known to produce cellulosomes, changes the composition of its cellulosomes depending on the growth substrates. However, studies have investigated only cellulosomal proteins; profile changes in noncellulosomal proteins have rarely been examined. In this study, we performed a quantitative proteome analysis of the whole exoproteome of C. cellulovorans, including cellulosomal and noncellulosomal proteins, to illustrate how various substrates are efficiently degraded. C. cellulovorans was cultured with cellobiose, xylan, pectin, or phosphoric acid-swollen cellulose (PASC) as the sole carbon source. PASC was used as a cellulose substrate for more accurate quantitative analysis. Using an isobaric tag method and a liquid chromatography mass spectrometer equipped with a long monolithic silica capillary column, 639 proteins were identified and quantified in all 4 samples. Among these, 79 proteins were involved in saccharification, including 35 cellulosomal and 44 noncellulosomal proteins. We compared protein abundance by spectral count and found that cellulosomal proteins were more abundant than noncellulosomal proteins. Next, we focused on the fold change of the proteins depending on the growth substrates. Drastic changes were observed mainly among the noncellulosomal proteins. These results indicate that cellulosomal proteins were primarily produced to efficiently degrade any substrate and that noncellulosomal proteins were specifically produced to optimize the degradation of a particular substrate. This study highlights the importance of noncellulosomal proteins as well as cellulosomes for the efficient degradation of various substrates.
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