ReviewPlanta2013
Progress in plant protoplast research.
Review in Planta, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
61 citing papers in PubMed.
- An effective method for isolation and regeneration of Solanum tuberosum mesophyll protoplasts for transgene-free genome editing.Journal, genetic engineering & biotechnology · 2026Article
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Comprehensive transcriptomic and functional characterization of protoplast regeneration in Angelica gigas Nakai.BMC plant biology · 2026Article
- Effect of Suberoylanilide Hydroxamic Acid and Phytosulfokine-Alpha on Successful Plant Regeneration from Embryogenic Callus-Derived Protoplasts of Garlic (International journal of molecular sciences · 2025Article
- Impedance flow cytometry for rapid quality assessment of protoplast cultures.Plant methods · 2025Article
- Using a Supramolecular Approach to Engineer Modular Hydrogel Platforms for Culturing Protoplasts - from General Tissue Engineering to Cellular Agriculture.Advanced biology · 2025Article
- Phenotype microarray-based assessment of metabolic variability in plant protoplasts.Plant methods · 2025Article
- A Detailed Protocol for the Isolation of Arabidopsis thaliana Mesophyll Protoplasts and their Applications.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Isolation and transformation of perennial ryegrass (Frontiers in plant science · 2025Article
- Development of an optimized protocol for protoplast-to-plant regeneration of selected varieties of Brassica oleracea L.BMC plant biology · 2024Article
- Optimization of Protoplast Preparation Conditions inJournal of fungi (Basel, Switzerland) · 2024Article
- OptimizingPlants (Basel, Switzerland) · 2024Review
- A Comparison of DNA-Methylation during Protoplast Culture of Ponkan Mandarin (Plants (Basel, Switzerland) · 2024Article
- Direct protein delivery into intact Arabidopsis cells for genome engineering.Scientific reports · 2024Article
- Modern Technologies Provide New Opportunities for Somatic Hybridization in the Breeding of Woody Plants.Plants (Basel, Switzerland) · 2024Review
- Wheat Cybrid Plants, OryzaWheat, Regenerated from Wheat-Rice Hybrid Zygotes via in Vitro Fertilization System Possess Wheat-Rice Hybrid Mitochondria.Plant & cell physiology · 2024Article
- Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli.Genes · 2024Review
- Optimization of preparation conditions for Salsola laricifolia protoplasts using response surface methodology and artificial neural network modeling.Plant methods · 2024Article
- Breeding of ornamental orchids with focus on Phalaenopsis: current approaches, tools, and challenges for this century.Heredity · 2024Review
- Immunodetection of Cell Wall Components in Studies on Cell Wall Rebuilding in Fagopyrum esculentum and Fagopyrum tataricum.Methods in molecular biology (Clifton, N.J.) · 2024Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this review we focus on recent progress in protoplast regeneration, symmetric and asymmetric hybridization and novel technology developments. Regeneration of new species and improved culture techniques opened new horizons for practical breeding in a number of crops. The importance of protoplast sources and embedding systems is discussed. The study of reactive oxygen species effects and DNA (de)condensation, along with thorough phytohormone monitoring, are in our opinion the most promising research topics in the further strive for rationalization of protoplast regeneration. Following, fusion and fragmentation progress is summarized. Genomic, transcriptomic and proteomic studies have led to better insights in fundamental processes such as cell wall formation, cell development and chromosome rearrangements in fusion products, whether or not obtained after irradiation. Advanced molecular screening methods of both genome and cytoplasmome facilitate efficient screening of both symmetric and asymmetric fusion products. We expect that emerging technologies as GISH, high resolution melting and next generation sequencing will pay major contributions to our insights of genome creation and stabilization, mainly after asymmetric hybridization. Finally, we demonstrate agricultural valorization of somatic hybridization through enumerating recent introgression of diverse traits in a number of commercial crops.
Indexed as
Identifiers
23955146What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.