ArticleJournal of bacteriology2013
Physiological and transcriptional responses to osmotic stress of two Pseudomonas syringae strains that differ in epiphytic fitness and osmotolerance.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 71 citations in OpenAlex.
- Effects of sublethal stress application on the survival of bacterial inoculants: a systematic review.Archives of microbiology · 2023Pooled it
- To Beet or Not (Just) to Beet: Ecology, Virulence and Genomic Insights Into the Leaf Spot Disease Causative Pseudomonas syringae pv. aptata.Molecular plant pathology · 2026Review
- Pseudomonads associated to salt-stressed plants facilitate stress adaption of soybean through enhanced lignin biosynthesis.Science advances · 2026Article
- The LuxS/AI-2 quorum sensing system regulates osmotic adaptation, motility, and type VI secretion system inFrontiers in microbiology · 2026Article
- Differential hypo-osmotic stress responses and regulatory mechanisms ofApplied and environmental microbiology · 2025Article
- Complete genome sequence ofMicrobiology resource announcements · 2025Article
- Understanding the Impact of Salt Stress on Plant Pathogens Through Phenotypic and Transcriptomic Analysis.Plants (Basel, Switzerland) · 2025Article
- Article
- The role of the type VI secretion system in the stress resistance of plant-associated bacteria.Stress biology · 2024Review
- Transcriptome profiling of type VI secretion system core geneMicrobiology spectrum · 2024Article
- Application of Plant Growth-Promoting Bacteria from Cape Verde to Increase Maize Tolerance to Salinity.Antioxidants (Basel, Switzerland) · 2023Article
- Dynamic character displacement among a pair of bacterial phyllosphere commensals in situ.Nature communications · 2022Article
- Pseudomonas syringae addresses distinct environmental challenges during plant infection through the coordinated deployment of polysaccharides.Journal of experimental botany · 2022Article
- Beyond dueling: roles of the type VI secretion system in microbiome modulation, pathogenesis and stress resistance.Stress biology · 2021Review
- Regulation of theMicroorganisms · 2021Review
- Genome-wide gene expression changes of Pseudomonas veronii 1YdBTEX2 during bioaugmentation in polluted soils.Environmental microbiome · 2021Article
- Trehalose and α-glucan mediate distinct abiotic stress responses in Pseudomonas aeruginosa.PLoS genetics · 2021Article
- Rhizosphere plant-microbe interactions under water stress.Advances in applied microbiology · 2021Article
- LeveragingFrontiers in microbiology · 2021Review
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The foliar pathogen Pseudomonas syringae is a useful model for understanding the role of stress adaptation in leaf colonization. We investigated the mechanistic basis of differences in the osmotolerance of two P. syringae strains, B728a and DC3000. Consistent with its higher survival rates following inoculation onto leaves, B728a exhibited superior osmotolerance over DC3000 and higher rates of uptake of plant-derived osmoprotective compounds. A global transcriptome analysis of B728a and DC3000 following an osmotic upshift demonstrated markedly distinct responses between the strains; B728a showed primarily upregulation of genes, including components of the type VI secretion system (T6SS) and alginate biosynthetic pathways, whereas DC3000 showed no change or repression of orthologous genes, including downregulation of the T3SS. DC3000 uniquely exhibited improved growth upon deletion of the biosynthetic genes for the compatible solute N-acetylglutaminylglutamine amide (NAGGN) in a minimal medium, due possibly to NAGGN synthesis depleting the cellular glutamine pool. Both strains showed osmoreduction of glnA1 expression, suggesting that decreased glutamine synthetase activity contributes to glutamate accumulation as a compatible solute, and both strains showed osmoinduction of 5 of 12 predicted hydrophilins. Collectively, our results demonstrate that the superior epiphytic competence of B728a is consistent with its strong osmotolerance, a proactive response to an osmotic upshift, osmoinduction of alginate synthesis and the T6SS, and resiliency of the T3SS to water limitation, suggesting sustained T3SS expression under the water-limited conditions encountered during leaf colonization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.