ArticleDiagnostic microbiology and infectious disease2014
Use of quantitative 16S rRNA PCR to determine bacterial load does not augment conventional cerebrospinal fluid (CSF) cultures among children undergoing treatment for CSF shunt infection.
Article in Diagnostic microbiology and infectious disease, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Evaluation of 415-nm Blue Light and Hypochlorous Acid on the Rhesus Macaque (Macaca mulatta) Cranial Implant Microbiota.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- A search for bacteria identified from cerebrospinal fluid shunt infections in previous surgical events.PloS one · 2024Article
- A Refined, Controlled 16S rRNA Gene Sequencing Approach Reveals Limited Detection of Cerebrospinal Fluid Microbiota in Children with Bacterial Meningitis.Microbiology spectrum · 2023Article
- Characterization of cerebrospinal fluid (CSF) microbiota at the time of initial surgical intervention for children with hydrocephalus.PloS one · 2023Article
- Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sites.BMC microbiology · 2022Article
- A Novel Adult Murine Model of Typical EnteroaggregativeFrontiers in cellular and infection microbiology · 2022Article
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- Molecular Characterization of Microbiota in Cerebrospinal Fluid From Patients With CSF Shunt Infections Using Whole Genome Amplification Followed by Shotgun Sequencing.Frontiers in cellular and infection microbiology · 2021Article
- Differences in airway microbiome and metabolome of single lung transplant recipients.Respiratory research · 2020Article
- Changing the Paradigm of Research in Pediatric Hospital Medicine.Hospital pediatrics · 2019Article
- Cerebrospinal Fluid Shunt Infection: Emerging Paradigms in Pathogenesis that Affect Prevention and Treatment.The Journal of pediatrics · 2019Article
- CSF inflammatory markers differ in gram-positive versus gram-negative shunt infections.Journal of neuroinflammation · 2019Article
- Detection of 16S rRNA and KPC Genes from Complex Matrix Utilizing a Molecular Inversion Probe Assay for Next-Generation Sequencing.Scientific reports · 2018Article
- How low can we go? The implications of low bacterial load in respiratory microbiota studies.Pneumonia (Nathan Qld.) · 2018Review
- Malnutrition in HIV-Infected Children Is an Indicator of Severe Disease with an Impaired Response to Antiretroviral Therapy.AIDS research and human retroviruses · 2018Observational
- Routine testing for anaerobic bacteria in cerebrospinal fluid cultures improves recovery of clinically significant pathogens.Journal of clinical microbiology · 2014Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
The aim of this study was to develop a quantitative 16S rRNA assay for determination of bacterial nucleic acid load in cerebrospinal fluid (CSF) shunt infection and to compare quantitative 16S rRNA polymerase chain reaction (PCR) findings to those of conventional bacterial culture in patients treated for CSF shunt infection. We developed a quantitative 16S rRNA PCR assay that detected bacterial load across a range of 2.5 × 10(9) down to 2.5 × 10(4) 16S copies/mL CSF under experimental conditions for numerous Gram-positive and Gram-negative organisms. However, when applied to archived CSF samples from 25 shunt infection episodes, correlations between positive bacterial culture and 16S rRNA levels were seen in only half of infections, and 16S rRNA levels dropped precipitously after an initial peak on the first day of sample collection. Bacterial load measured using 16S rRNA PCR does not provide sufficient information beyond bacterial culture to inform CSF shunt infection treatment.
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