ArticleTissue engineering. Part A2014
Developmental-like bone regeneration by human embryonic stem cell-derived mesenchymal cells.
Article in Tissue engineering. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 61 citations in OpenAlex.
- Start, Stop, Rewind, Repeat-Cyclic Exposure of Adipose Stromal Cells-derived Cartilage Organoids to Chondrogenic and Proliferative Cues to Achieve Scaled-up and Customizable Bone Formation by Endochondral Ossification.Advanced healthcare materials · 2026Article
- Posttranscriptional Modification to Modulate Progenitor Differentiation on Heterotypic Spheroids.Tissue engineering. Part A · 2024Article
- Mesenchymal stromal cells for bone trauma, defects, and disease: Considerations for manufacturing, clinical translation, and effective treatments.Bone reports · 2023Article
- iPSC-neural crest derived cells embedded in 3D printable bio-ink promote cranial bone defect repair.Scientific reports · 2022Article
- Bioengineered Living Bone Grafts-A Concise Review on Bioreactors and Production Techniques In Vitro.International journal of molecular sciences · 2022Review
- A Narrative Review of Cell-Based Approaches for Cranial Bone Regeneration.Pharmaceutics · 2022Review
- Cell-based therapies for rheumatoid arthritis: opportunities and challenges.Therapeutic advances in musculoskeletal disease · 2022Review
- miRNA induced co-differentiation and cross-talk of adipose tissue-derived progenitor cells for 3D heterotypic pre-vascularized bone formation.Biofabrication · 2021Article
- Novel cell sources for bone regeneration.MedComm · 2021Review
- Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) into Osteoclasts.Bio-protocol · 2020Article
- Biodegradable materials for bone defect repair.Military Medical Research · 2020Review
- Collagen-infilled 3D printed scaffolds loaded with miR-148b-transfected bone marrow stem cells improve calvarial bone regeneration in rats.Materials science & engineering. C, Materials for biological applications · 2019Article
- Induced Pluripotent Stem Cell-Derived Mesenchymal Stromal Cells Are Functionally and Genetically Different From Bone Marrow-Derived Mesenchymal Stromal Cells.Stem cells (Dayton, Ohio) · 2019Article
- Current advances for bone regeneration based on tissue engineering strategies.Frontiers of medicine · 2019Review
- Human iPSC-derived MSCs (iMSCs) from aged individuals acquire a rejuvenation signature.Stem cell research & therapy · 2019Article
- Tissue Engineering for the Temporomandibular Joint.Advanced healthcare materials · 2019Review
- A biomaterial with a channel-like pore architecture induces endochondral healing of bone defects.Nature communications · 2018Article
- Antimicrobial Peptide Combined with BMP2-Modified Mesenchymal Stem Cells Promotes Calvarial Repair in an Osteolytic Model.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Article
- Craniometaphyseal Dysplasia Mutations in ANKH Negatively Affect Human Induced Pluripotent Stem Cell Differentiation into Osteoclasts.Stem cell reports · 2017Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
The in vivo osteogenesis potential of mesenchymal-like cells derived from human embryonic stem cells (hESC-MCs) was evaluated in vivo by implantation on collagen/hydroxyapatite scaffolds into calvarial defects in immunodeficient mice. This study is novel because no osteogenic or chondrogenic differentiation protocols were applied to the cells prior to implantation. After 6 weeks, X-ray, microCT, and histological analysis showed that the hESC-MCs had consistently formed a highly vascularized new bone that bridged the bone defect and seamlessly integrated with host bone. The implanted hESC-MCs differentiated in situ to functional hypertrophic chondrocytes, osteoblasts, and osteocytes forming new bone tissue via an endochondral ossification pathway. Evidence for the direct participation of the human cells in bone morphogenesis was verified by two separate assays: with Alu and by human mitochondrial antigen positive staining in conjunction with co-localized expression of human bone sialoprotein in histologically verified regions of new bone. The large volume of new bone in a calvarial defect and the direct participation of the hESC-MCs far exceeds that of previous studies and that of the control adult hMSCs. This study represents a key step forward for bone tissue engineering because of the large volume, vascularity, and reproducibility of new bone formation and the discovery that it is advantageous to not over-commit these progenitor cells to a particular lineage prior to implantation. The hESC-MCs were able to recapitulate the mesenchymal developmental pathway and were able to repair the bone defect semi-autonomously without preimplantation differentiation to osteo- or chondroprogenitors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.