ArticleAnalytical chemistry2013
Platform for high-throughput testing of the effect of soluble compounds on 3D cell cultures.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 121 citations in OpenAlex.
- Spheromatrix: a paper-based platform for scalable 3D tumor model generation, cryopreservation, and high-throughput drug assessment.Microsystems & nanoengineering · 2025Article
- Microfluidic Technologies in Advancing Cancer Research.Micromachines · 2024Review
- Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications.Bioengineering & translational medicine · 2024Review
- Combinatorial Microgels for 3D ECM Screening and Heterogeneous Microenvironmental Culture of Primary Human Hepatic Stellate Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Combinatorial Microgels for 3D ECM Screening and Heterogeneous Microenvironmental Culture of Primary Human Hepatic Stellate Cells.bioRxiv : the preprint server for biology · 2023Article
- Multi-Layer Nanofibrous PCL Scaffold-Based Colon Cancer Cell Cultures to Mimic Hypoxic Tumor Microenvironment for Bioassay.Cancers · 2021Article
- Low magnitude high frequency vibrations expedite the osteogenesis of bone marrow stem cells on paper based 3D scaffolds.Biomedical engineering letters · 2020Article
- Establishment of 2.5D organoid culture model using 3D bladder cancer organoid culture.Scientific reports · 2020Article
- Paper-Supported High-Throughput 3D Culturing, Trapping, and Monitoring of Caenorhabditis Elegans.Micromachines · 2020Article
- Generating linear oxygen gradients across 3D cell cultures with block-layered oxygen controlled chips (BLOCCs).Analytical methods : advancing methods and applications · 2020Article
- Applications of paper as a support material in biomedical sciences: A decadal review.Sensors international · 2020Review
- Tissue Papers: Leveraging Paper-Based Microfluidics for the Next Generation of 3D Tissue Models.Analytical chemistry · 2019Article
- Paper-based Transwell assays: an inexpensive alternative to study cellular invasion.The Analyst · 2018Article
- Screening Estrogen Receptor Modulators in a Paper-Based Breast Cancer Model.Analytical chemistry · 2018Article
- Unmasking molecular profiles of bladder cancer.Investigative and clinical urology · 2018Review
- A versatile 3D tissue matrix scaffold system for tumor modeling and drug screening.Science advances · 2017Article
- Article
- The potential of organoids in urological cancer research.Nature reviews. Urology · 2017Review
- Understanding the cancer/tumor biology from 2D to 3D.Journal of thoracic disease · 2016Article
- Osteogenic differentiation of preosteoblasts on a hemostatic gelatin sponge.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
In vitro 3D culture could provide an important model of tissues in vivo, but assessing the effects of chemical compounds on cells in specific regions of 3D culture requires physical isolation of cells and thus currently relies mostly on delicate and low-throughput methods. This paper describes a technique ("cells-in-gels-in-paper", CiGiP) that permits rapid assembly of arrays of 3D cell cultures and convenient isolation of cells from specific regions of these cultures. The 3D cultures were generated by stacking sheets of 200-μm-thick paper, each sheet supporting 96 individual "spots" (thin circular slabs) of hydrogels containing cells, separated by hydrophobic material (wax, PDMS) impermeable to aqueous solutions, and hydrophilic and most hydrophobic solutes. A custom-made 96-well holder isolated the cell-containing zones from each other. Each well contained media to which a different compound could be added. After culture and disassembly of the holder, peeling the layers apart "sectioned" the individual 3D cultures into 200-μm-thick sections which were easy to analyze using 2D imaging (e.g., with a commercial gel scanner). This 96-well holder brings new utilities to high-throughput, cell-based screening, by combining the simplicity of CiGiP with the convenience of a microtiter plate. This work demonstrated the potential of this type of assays by examining the cytotoxic effects of phenylarsine oxide (PAO) and cyclophosphamide (CPA) on human breast cancer cells positioned at different separations from culture media in 3D cultures.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.