ArticlePloS one2013
Leukocyte proliferation and immune modulator production in patients with chronic kidney disease.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Hepatitis B virus vaccine immune response and mortality in dialysis patients: a meta-analysis.Journal of nephrology · 2020Pooled it
- Vitamin D receptor activation reduces inflammatory cytokines and plasma MicroRNAs in moderate chronic kidney disease - a randomized trial.BMC nephrology · 2017Trial
- Immunomodulatory effect of CD71BMC nephrology · 2025Article
- Assessment of Eotaxin Concentration in Children with Chronic Kidney Disease.International journal of molecular sciences · 2025Article
- In Vitro Study of Vitamin D Effects on Immune, Endothelial, and Vascular Smooth Muscle Cells in Chronic Kidney Disease.International journal of molecular sciences · 2025Article
- Residual Kidney Function and Cause-Specific Mortality Among Incident Hemodialysis Patients.Kidney international reports · 2023Article
- Application of regularized regression to identify novel predictors of mortality in a cohort of hemodialysis patients.Scientific reports · 2021Article
- Muscle-Bone Crosstalk in Chronic Kidney Disease: The Potential Modulatory Effects of Exercise.Calcified tissue international · 2021Review
- Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney Disease.Journal of clinical medicine · 2020Article
- AIDS incidence trends at presentation and during follow-up among HIV-at-risk populations: a 15-year nationwide cohort study in Taiwan.BMC public health · 2018Article
- Disseminated Cryptococcosis with Rapidly Growing Lung Nodules in an End-stage Renal Disease Patient.Internal medicine (Tokyo, Japan) · 2017Article
- The Changing Landscape of Renal Inflammation.Trends in molecular medicine · 2016Review
- Article
- Activation of basophils is a new and sensitive marker of biocompatibility in hemodialysis.Artificial organs · 2014Article
- Toll-like receptor activation: from renal inflammation to fibrosis.Kidney international supplements · 2014Review
- Noncardiovascular mortality in CKD: an epidemiological perspective.Nature reviews. Nephrology · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionIn Chronic Kidney Disease (CKD), immune cells are affected by uremic retention toxins. Given this effect, we analyzed lymphocyte proliferative response and immune modulators production following in vitro stimulation.
methodsWhole blood was drawn from healthy controls, patients with eGFR <20 ml/min/1.73 m(2) (Pre-dialysis, CKD stages 4 and 5) and hemodialysis patients (stage 5D). Peripheral cells were incubated for six days with pokeweed mitogen, concanavalin A, Staphylococcus enterotoxin A or influenza A vaccine. Peripheral lymphocyte proliferation was then analyzed by the "Flow-cytometric Assay of Specific Cell-mediated Immune response in Activated whole blood" (FASCIA) method, and cytokine profile in the cell supernatants was analyzed by the Milliplex multi-array method.
resultsThe absolute number of lymphoblasts in response to mitogenic stimulation and the number of cells in each CD4+ and CD8+ subpopulation were similar comparing the three groups, except for a single decline in number of lymphoblasts after stimulation with Staphylococcus enterotoxin A, comparing dialysis patients with healthy controls. Levels of interleukin (IL)-2 (p=0.026), -10 (p=0.019) and -15 (p=0.027) in the Staphylococcus enterotoxin A-stimulated supernatant were lower in hemodialysis patients compared to healthy controls. Levels of IL-15 (p=0.017) from pre-dialysis patients and levels of IL-5 (p=0.019) from hemodialysis patients in influenza A vaccine-stimulated supernatants were also lower compared to controls. In pokeweed mitogen-stimulated supernatant, IL-2 levels (p=0.013) were lower in hemodialysis patients compared to pre-dialysis patients. TNF-α, IL-10, IL-12, IL-15, IL-8, MCP-1, IP-10, IFN-α2, IL-1α and eotaxin levels were all significantly higher in plasma obtained from CKD patients.
conclusionOur results suggest that T-cells from CKD patients have similar proliferative response to stimulation compared with healthy individuals. Moreover, however the immune cells show inability to produce selected cytokines, most likely due to the uremic milieu or dialysis procedure.
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