ArticlePloS one2013
Induced expression of AtLEC1 and AtLEC2 differentially promotes somatic embryogenesis in transgenic tobacco plants.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 85 citations in OpenAlex.
- The multifaceted role of gibberellins in somatic embryogenesis: fromFrontiers in plant science · 2026Review
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- Functional analysis of the woody oil crop Plukenetia volubilis L. LEC2 homolog PvoB3-69 in promoting regeneration.Plant cell reports · 2025Article
- Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Plants (Basel, Switzerland) · 2025Article
- Functional Mechanisms and the Application of Developmental Regulators for Improving Genetic Transformation in Plants.Plants (Basel, Switzerland) · 2024Review
- Appreciating animal induced pluripotent stem cells to shape plant cell reprogramming strategies.Journal of experimental botany · 2024Review
- An inducible CRISPR activation tool for accelerating plant regeneration.Plant communications · 2024Article
- Application of Developmental Regulators for Enhancing Plant Regeneration and Genetic Transformation.Plants (Basel, Switzerland) · 2024Review
- The improvement of the in vitro plant regeneration in barley with the epigenetic modifier of histone acetylation, trichostatin A.Journal of applied genetics · 2024Article
- Epigenetic modifications and miRNAs determine the transition of somatic cells into somatic embryos.Plant cell reports · 2023Review
- Advances in Somatic Embryogenesis of Banana.International journal of molecular sciences · 2023Review
- RWP-RK Domain 3 (OsRKD3) induces somatic embryogenesis in black rice.BMC plant biology · 2023Article
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- Multifaceted roles of transcription factors during plant embryogenesis.Frontiers in plant science · 2023Review
- GRAS transcription factors emerging regulator in plants growth, development, and multiple stresses.Molecular biology reports · 2022Review
- Tissue Culture and Somatic Embryogenesis in Warm-Season Grasses-Current Status and Its Applications: A Review.Plants (Basel, Switzerland) · 2022Review
- Internal and External Regulatory Elements Controlling Somatic Embryogenesis in Catharanthus: A Model Medicinal Plant.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Molecular Determinants ofFrontiers in plant science · 2022Review
- Review
- Genetic and Molecular Control of Somatic Embryogenesis.Plants (Basel, Switzerland) · 2021Review
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabidopsis LEAFY COTYLEDON (LEC) genes, AtLEC1 and AtLEC2, are important embryonic regulators that play key roles in morphogenesis and maturation phases during embryo development. Ectopic expression of AtLEC1 and AtLEC2 in tobacco caused abnormality in transgenic seedling. When transgenic seeds germinated on medium containing 30 µM DEX, LEC1 transgenic seedlings were ivory and fleshy, with unexpanded cotyledons, stubby hypocotyls, short roots and no obvious callus formation at the shoot meristem position. While LEC2 transgenic seedlings formed embryonic callus on the shoot apical meristem and somatic embryo-like structures emerged from the surface of the callus. When callus were transferred to hormone free MS0 medium more shoots were regenerated from each callus. However, shoot formation was not observed in LEC1 overexpressors. To investigate the mechanisms of LEC2 in somatic embryogenesis, we studied global gene expression by digital gene expression profiling analysis. The results indicated that ectopic expression of LEC2 genes induced accumulation of embryo-specific proteins such as seed storage proteins, late embryogenesis abundant (LEA) proteins, fatty acid biosynthetic enzymes, products of steroid biosynthesis related genes and key regulatory genes of the embryo development. Genes of plant-specific transcription factors such as NAC domain protein, AP2 and GRAS family, resistance-related as well as salicylic acid signaling related genes were up-regulated in LEC2 transgenic seedlings. Ectopi c expression of LEC2 induced large number of somatic embryo formation and shoot regeneration but 20 d DEX induction of LEC1 is not sufficient to induce somatic embryogenesis and shoot formation. Our data provide new information to understand the mechanisms on LEC2 gene's induction of somatic embryogenesis.
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