ArticlePloS one2013
Genomic and proteomic analysis of Schizaphis graminum reveals cyclophilin proteins are involved in the transmission of cereal yellow dwarf virus.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 64 citations in OpenAlex.
- Rewiring the proteome of the Euscelidius variegatus holobiont in response to Flavescence dorée phytoplasma.Scientific reports · 2025Article
- Chromosome-level genome assembly of vetch aphid Megoura crassicauda (Hemiptera: Aphididae).Scientific data · 2025Article
- Review
- Genetic diversity in vector populations influences the transmission efficiency of an important plant virus.Biology letters · 2024Article
- Comparative evolutionary analyses of eight whitefly Bemisia tabaci sensu lato genomes: cryptic species, agricultural pests and plant-virus vectors.BMC genomics · 2023Article
- A New Perspective on the Co-Transmission of Plant Pathogens by Hemipterans.Microorganisms · 2023Review
- Differential gene expression between viruliferous and non-viruliferous Schizaphis graminum (Rondani).PloS one · 2023Article
- Sublethal effects of imidacloprid on the fitness of two species of wheat aphids, Schizaphis graminum (R.) and Rhopalosiphum padi (L.).PloS one · 2023Article
- Comprehensive analysis of lysine lactylation inFrontiers in genetics · 2022Article
- Combined Transcriptomic and Proteomic Analysis ofInsects · 2021Article
- A proteomic approach reveals possible molecular mechanisms and roles for endosymbiotic bacteria in begomovirus transmission by whiteflies.GigaScience · 2020Article
- Applications of Proteomic Tools to Study Insect Vector-Plant Virus Interactions.Life (Basel, Switzerland) · 2020Review
- Discovery of Novel Thrips Vector Proteins That Bind to the Viral Attachment Protein of the Plant Bunyavirus Tomato Spotted Wilt Virus.Journal of virology · 2019Article
- Plant-Mediated Silencing of the WhiteflyFrontiers in physiology · 2019Article
- Identification of Plant Virus Receptor Candidates in the Stylets of Their Aphid Vectors.Journal of virology · 2018Article
- Article
- Host Plants Indirectly Influence Plant Virus Transmission by Altering Gut Cysteine Protease Activity of Aphid Vectors.Molecular & cellular proteomics : MCP · 2017Article
- Plant Virus-Insect Vector Interactions: Current and Potential Future Research Directions.Viruses · 2016Review
- Implication of the WhiteflyFrontiers in plant science · 2016Article
- Proteomic Analysis of Interaction between a Plant Virus and Its Vector Insect Reveals New Functions of Hemipteran Cuticular Protein.Molecular & cellular proteomics : MCP · 2015Article
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Yellow dwarf viruses cause the most economically important virus diseases of cereal crops worldwide and are transmitted by aphid vectors. The identification of aphid genes and proteins mediating virus transmission is critical to develop agriculturally sustainable virus management practices and to understand viral strategies for circulative movement in all insect vectors. Two cyclophilin B proteins, S28 and S29, were identified previously in populations of Schizaphisgraminum that differed in their ability to transmit the RPV strain of Cereal yellow dwarf virus (CYDV-RPV). The presence of S29 was correlated with F2 genotypes that were efficient virus transmitters. The present study revealed the two proteins were isoforms, and a single amino acid change distinguished S28 and S29. The distribution of the two alleles was determined in 12 F2 genotypes segregating for CYDV-RPV transmission capacity and in 11 genetically independent, field-collected S. graminum biotypes. Transmission efficiency for CYDV-RPV was determined in all genotypes and biotypes. The S29 isoform was present in all genotypes or biotypes that efficiently transmit CYDV-RPV and more specifically in genotypes that efficiently transport virus across the hindgut. We confirmed a direct interaction between CYDV-RPV and both S28 and S29 using purified virus and bacterially expressed, his-tagged S28 and S29 proteins. Importantly, S29 failed to interact with a closely related virus that is transported across the aphid midgut. We tested for in vivo interactions using an aphid-virus co-immunoprecipitation strategy coupled with a bottom-up LC-MS/MS analysis using a Q Exactive mass spectrometer. This analysis enabled us to identify a third cyclophilin protein, cyclophilin A, interacting directly or in complex with purified CYDV-RPV. Taken together, these data provide evidence that both cyclophilin A and B interact with CYDV-RPV, and these interactions may be important but not sufficient to mediate virus transport from the hindgut lumen into the hemocoel.
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