ArticlePloS one2013
Sirt1 inhibits akt2-mediated porcine adipogenesis potentially by direct protein-protein interaction.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Role of sirtuins in obesity and osteoporosis: molecular mechanisms and therapeutic targets.Cell communication and signaling : CCS · 2025Review
- Antiobesity Drug Discovery Research:Current drug targets · 2024Review
- Guanidinoacetic Acid Attenuates Adipogenesis through Regulation of miR-133a in Sheep.Animals : an open access journal from MDPI · 2023Article
- Activation of LRP6 with HLY78 Attenuates Oxidative Stress and Neuronal Apoptosis via GSK3Oxidative medicine and cellular longevity · 2022Article
- Review
- miR-29a Modulates GSK3β/SIRT1-Linked Mitochondrial Proteostatic Stress to Ameliorate Mouse Non-Alcoholic Steatohepatitis.International journal of molecular sciences · 2020Article
- mAutophagy · 2020Article
- Antiobesity Effects of Extract fromNutrients · 2020Article
- Obesity May Accelerate the Aging Process.Frontiers in endocrinology · 2019Review
- miR-425-5p Inhibits Differentiation and Proliferation in Porcine Intramuscular Preadipocytes.International journal of molecular sciences · 2017Article
- Deciphering the regulation of porcine genes influencing growth, fatness and yield-related traits through genetical genomics.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Article
- Sirt1 decreased adipose inflammation by interacting with Akt2 and inhibiting mTOR/S6K1 pathway in mice.Journal of lipid research · 2016Article
- Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review.International journal of molecular sciences · 2016Review
- Circulating SIRT1 Increases After Intragastric Balloon Fat Loss in Obese Patients.Obesity surgery · 2016Article
- Expression Profiles of IGF-1R Gene and Polymorphisms of its Regulatory Regions in Different Pig Breeds.The protein journal · 2016Article
- Lipogenesis in myoblasts and its regulation of CTRP6 by AdipoR1/Erk/PPARγ signaling pathway.Acta biochimica et biophysica Sinica · 2016Article
- Moderately decreased maternal dietary energy intake during pregnancy reduces fetal skeletal muscle mitochondrial biogenesis in the pigs.Genes & nutrition · 2016Article
- Green tea extracts reduce adipogenesis by decreasing expression of transcription factors C/EBPα and PPARγ.International journal of clinical and experimental medicine · 2014Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Compared with the rodent, the domestic pig is a much better animal model for studying adipogenesis and obesity-related diseases. Currently, the role of Akt2 and Sirt1 in porcine adipogenesis remains elusive. In this study, we defined the effect of Akt2 and Sirt1 on porcine preadipocyte lipogenesis and the regulatory mechanism. First, we found that Akt2 was widely expressed in porcine various tissues and at high level in adipose tissue. Further analysis showed that the expression level of Akt2 was much higher in adipose tissue and adipocytes of the Bamei pig breed (a Chinese indigenous fatty pig) than in that of the Large White pig breed (a Lean type pig), whereas the level of Sirt1 expression was opposite. The expression levels of Sirt1 and Akt2 gradually increased during adipogenic differentiation. Adipogenesis was robustly inhibited in Akt2 deficient fat cells, whereas it was promoted in Sirt1 deficient cells using the lentiviral-mediated shRNA approach. Interestingly, adipogenesis returned to normal in Akt2 and Sirt1 dual-deficient cells, showing that the pro- and anti-adipogenic effects were balanced. Sirt1 inhibited transcriptional activity of Akt2 in a dose-dependent way. Interaction of endogenous Akt2 and Sirt1 was gradually enhanced before day 6 of differentiation, and then attenuated. Akt2 and Sirt1 also interacted with C/EBPα in adipocytes. Moreover, knockdown of Akt2 or/and Sirt1 affected pro-lipogenesis of insulin-stimulated by PI3K/Akt pathway. We further found that Sirt1 respectively interacted with PI3K and GSK3β which were key upstream and downstream components of PI3K/Akt pathway. Based on the above findings, we concluded that the crosstalk between C/EBPα and PI3K/Akt signaling pathways is implicated in Akt2 and Sirt1 regulation of adipogenesis.
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