ArticlePloS one2013
De novo assembly and characterization of the transcriptome, and development of SSR markers in wax gourd (Benicasa hispida).
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 54 citations in OpenAlex.
- Genome-wide identification of the GATA transcription factor family in ten Cucurbitaceae species and expression analysis of ClGATA genes in watermelon stress responses.BMC genomics · 2026Article
- A nonsynonymous mutation in BhLS, encoding an acyl-CoA N-acyltransferase leads to fruit and seed size variation in wax gourd (Benincasa hispida).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- De novo transcriptomic analysis of Doum Palm (Hyphaene compressa) revealed an insight into its potential drought tolerance.PloS one · 2024Article
- Screening and Verification of Reference Genes for Analysis of Gene Expression in Garlic (Plants (Basel, Switzerland) · 2023Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2022Article
- De-novo transcriptome analysis unveils differentially expressed genes regulating drought and salt stress response in Panicum sumatrense.Scientific reports · 2020Article
- Metabolic and transcriptomic analysis of two Cucurbita moschata germplasms throughout fruit development.BMC genomics · 2020Article
- The wax gourd genomes offer insights into the genetic diversity and ancestral cucurbit karyotype.Nature communications · 2019Article
- Comparative transcriptome and metabolite profiling of four tissues from Alisma orientale (Sam.) Juzep reveals its inflorescence developmental and medicinal characteristics.Scientific reports · 2019Article
- Transcriptome analysis and codominant markers development in caper, a drought tolerant orphan crop with medicinal value.Scientific reports · 2019Article
- RNA-seq-based analysis of the hypertrophic scarring with and without pressure therapy in a Bama minipig model.Scientific reports · 2018Article
- De novo assembly, characterization and annotation for the transcriptome of Sphaeroma terebrans and microsatellite marker discovery.Genes & genomics · 2018Article
- Article
- Transcriptome profiling of pumpkin (Cucurbita moschata Duch.) leaves infected with powdery mildew.PloS one · 2018Article
- A deletion of the gene encoding amino aldehyde dehydrogenase enhances the "pandan-like" aroma of winter melon (Benincasa hispida) and is a functional marker for the development of the aroma.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- Comparative Transcriptome Analysis Reveals Adaptive Evolution of Notopterygium incisum and Notopterygium franchetii, Two High-Alpine Herbal Species Endemic to China.Molecules (Basel, Switzerland) · 2017Article
- Article
- Transcriptome Profiling to Understand the Effect of Citrus Rootstocks on the Growth of 'Shatangju' Mandarin.PloS one · 2017Article
- De novo transcriptome sequencing and assembly from apomictic and sexual Eragrostis curvula genotypes.PloS one · 2017Article
- Temperature expression patterns of genes and their coexpression with LncRNAs revealed by RNA-Seq in non-heading Chinese cabbage.BMC genomics · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWax gourd is a widely used vegetable of Cucuribtaceae, and also has important medicinal and health values. However, the genomic resources of wax gourd were scarcity, and only a few nucleotide sequences could be obtained in public databases. METHODOLOGY/PRINCIPAL
findingsIn this study, we examined transcriptome in wax gourd. More than 44 million of high quality reads were generated from five different tissues of wax gourd using Illumina paired-end sequencing technology. Approximately 4 Gbp data were generated, and de novo assembled into 65,059 unigenes, with an N50 of 1,132 bp. Based on sequence similarity search with known protein database, 36,070 (55.4%) showed significant similarity to known proteins in Nr database, and 24,969 (38.4%) had BLAST hits in Swiss-Prot database. Among the annotated unigenes, 14,994 of wax gourd unigenes were assigned to GO term annotation, and 23,977 were found to have COG classifications. In addition, a total of 18,713 unigenes were assigned to 281 KEGG pathways. Furthermore, 6,242 microsatellites (simple sequence repeats) were detected as potential molecular markers in wax gourd. Two hundred primer pairs for SSRs were designed for validation of the amplification and polymorphism. The result showed that 170 of the 200 primer pairs were successfully amplified and 49 (28.8%) of them exhibited polymorphisms. CONCLUSION/SIGNIFICANCE: Our study enriches the genomic resources of wax gourd and provides powerful information for future studies. The availability of this ample amount of information about the transcriptome and SSRs in wax gourd could serve as valuable basis for studies on the physiology, biochemistry, molecular genetics and molecular breeding of this important vegetable crop.
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