ArticlePloS one2013
Evaluation of reference genes for RT qPCR analyses of structure-specific and hormone regulated gene expression in Physcomitrella patens gametophytes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 83 citations in OpenAlex.
- MAdLandExpression: integrating sexual reproduction into the Physcomitrium patens expression atlas.The Plant journal : for cell and molecular biology · 2026Article
- Essential developmental processes in Physcomitrium patens require distinct levels of total activity provided by functionally redundant PpROP GTPases.The New phytologist · 2025Article
- The role of auxin-mediated gene activation in the bryophyte, Physcomitrium patens.Journal of experimental botany · 2025Article
- Selection and Validation of Reference Genes for RT-qPCR in Protonemal Tissue of the Desiccation-Tolerant MossPlants (Basel, Switzerland) · 2025Article
- The genetic puzzle of multicopy genes: challenges and troubleshooting.Plant methods · 2025Article
- Transcriptional and functional characterization in the terpenoid precursor pathway of the early land plant Physcomitrium patens.Plant biology (Stuttgart, Germany) · 2025Article
- SUPPRESSOR OF MAX2 1-LIKE (SMXL) homologs are MAX2-dependent repressors of Physcomitrium patens growth.The Plant cell · 2024Article
- Conservation of the moss RNA editing factor PPR78 despite the loss of its known cytidine-to-uridine editing sites is explained by a hidden extra target.The Plant cell · 2024Article
- PpSCARECROW1 (PpSCR1) regulates leaf blade and mid-vein development in Physcomitrium patens.Plant molecular biology · 2024Article
- Clade-D auxin response factors regulate auxin signaling and development in the moss Physcomitrium patens.PLoS biology · 2023Article
- Moss PIEZO homologs have a conserved structure, are ubiquitously expressed, and do not affect general vacuole function.Plant signaling & behavior · 2022Article
- Stress-associated developmental reprogramming in moss protonemata by synthetic activation of the common symbiosis pathway.iScience · 2022Article
- Autophagy modulates growth and development in the mossFrontiers in plant science · 2022Article
- The moss-specific transcription factor PpERF24 positively modulates immunity against fungal pathogens inFrontiers in plant science · 2022Article
- Transcriptional profiling reveals conserved and species-specific plant defense responses during the interaction of Physcomitrium patens with Botrytis cinerea.Plant molecular biology · 2021Article
- Comprehensive analysis of the Ppatg3 mutant reveals that autophagy plays important roles in gametophore senescence in Physcomitrella patens.BMC plant biology · 2020Article
- Functional redundancy and divergence of β-carbonic anhydrases in Physcomitrella patens.Planta · 2020Article
- Characterisation of evolutionarily conserved key players affecting eukaryotic flagellar motility and fertility using a moss model.The New phytologist · 2020Article
- Functional expression and characterization of cinnamic acid 4-hydroxylase from the hornwort Anthoceros agrestis in Physcomitrella patens.Plant cell reports · 2020Article
- A Chloroplast COR413 Protein FromFrontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of the moss Physcomitrella patens as a model system to study plant development and physiology is rapidly expanding. The strategic position of P. patens within the green lineage between algae and vascular plants, the high efficiency with which transgenes are incorporated by homologous recombination, advantages associated with the haploid gametophyte representing the dominant phase of the P. patens life cycle, the simple structure of protonemata, leafy shoots and rhizoids that constitute the haploid gametophyte, as well as a readily accessible high-quality genome sequence make this moss a very attractive experimental system. The investigation of the genetic and hormonal control of P. patens development heavily depends on the analysis of gene expression patterns by real time quantitative PCR (RT qPCR). This technique requires well characterized sets of reference genes, which display minimal expression level variations under all analyzed conditions, for data normalization. Sets of suitable reference genes have been described for most widely used model systems including e.g. Arabidopsis thaliana, but not for P. patens. Here, we present a RT qPCR based comparison of transcript levels of 12 selected candidate reference genes in a range of gametophytic P. patens structures at different developmental stages, and in P. patens protonemata treated with hormones or hormone transport inhibitors. Analysis of these RT qPCR data using GeNorm and NormFinder software resulted in the identification of sets of P. patens reference genes suitable for gene expression analysis under all tested conditions, and suggested that the two best reference genes are sufficient for effective data normalization under each of these conditions.
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