ArticlePloS one2013
The tip of the tail needle affects the rate of DNA delivery by bacteriophage P22.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- High-resolution Cryo-EM Analysis of the Therapeutic Pseudomonas Phage Pa223.Journal of molecular biology · 2025Article
- Viral Genome Delivery Across Bacterial Cell Surfaces.Annual review of microbiology · 2024Review
- Renewed insights into Ackermannviridae phage biology and applications.Npj viruses · 2024Review
- Coinfecting phages impede each other's entry into the cell.Current biology : CB · 2024Article
- Integrative structural analysis ofResearch square · 2024Article
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- Molecular Architecture of Salmonella Typhimurium Virus P22 Genome Ejection Machinery.Journal of molecular biology · 2023Article
- High-resolution cryo-EM structure of theScience advances · 2022Article
- Morphological and Genetic Characterization ofViruses · 2022Article
- Cryo-EM structure of the periplasmic tunnel of T7 DNA-ejectosome at 2.7 Å resolution.Molecular cell · 2021Article
- Intravirion DNA Can Access the Space Occupied by the Bacteriophage P22 Ejection Proteins.Viruses · 2021Article
- Ecology, Structure, and Evolution ofAnnual review of virology · 2020Review
- Article
- Article
- Genes affecting progression of bacteriophage P22 infection in Salmonella identified by transposon and single gene deletion screens.Molecular microbiology · 2018Article
- Structure of a Bacterial Virus DNA-Injection Protein Complex Reveals a Decameric Assembly with a Constricted Molecular Channel.PloS one · 2016Article
- Structural Plasticity of the Protein Plug That Traps Newly Packaged Genomes in Podoviridae Virions.The Journal of biological chemistry · 2016Article
- Article
- Exploring the Balance between DNA Pressure and Capsid Stability in Herpesviruses and Phages.Journal of virology · 2015Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The P22-like bacteriophages have short tails. Their virions bind to their polysaccharide receptors through six trimeric tailspike proteins that surround the tail tip. These short tails also have a trimeric needle protein that extends beyond the tailspikes from the center of the tail tip, in a position that suggests that it should make first contact with the host's outer membrane during the infection process. The base of the needle serves as a plug that keeps the DNA in the virion, but role of the needle during adsorption and DNA injection is not well understood. Among the P22-like phages are needle types with two completely different C-terminal distal tip domains. In the phage Sf6-type needle, unlike the other P22-type needle, the distal tip folds into a "knob" with a TNF-like fold, similar to the fiber knobs of bacteriophage PRD1 and Adenovirus. The phage HS1 knob is very similar to that of Sf6, and we report here its crystal structure which, like the Sf6 knob, contains three bound L-glutamate molecules. A chimeric P22 phage with a tail needle that contains the HS1 terminal knob efficiently infects the P22 host, Salmonella enterica, suggesting the knob does not confer host specificity. Likewise, mutations that should abrogate the binding of L-glutamate to the needle do not appear to affect virion function, but several different other genetic changes to the tip of the needle slow down potassium release from the host during infection. These findings suggest that the needle plays a role in phage P22 DNA delivery by controlling the kinetics of DNA ejection into the host.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.