ArticlePloS one2013
Trypsin potentiates human fibrocyte differentiation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Highly ordered macroporous hydrogen-bonded organic frameworks based on small biocompatible molecules.Nature communications · 2025Article
- Prevalence and Mechanisms of Itch in Chronic Wounds: A Narrative Review.Journal of clinical medicine · 2025Review
- Article
- Tryptase and Exogenous Trypsin: Mechanisms and Ophthalmic Applications.Journal of inflammation research · 2023Review
- Crustacean Proteases and Their Application in Debridement.Tropical life sciences research · 2020Article
- Monocyte-to-macrophage switch reversibly impaired by Ibrutinib.Oncotarget · 2019Article
- The Role of Trypsin:Chymotrypsin in Tissue Repair.Advances in therapy · 2018Review
- Protease activated-receptor 2 is necessary for neutrophil chemorepulsion induced by trypsin, tryptase, or dipeptidyl peptidase IV.Journal of leukocyte biology · 2018Article
- The Development of Serum Amyloid P as a Possible Therapeutic.Frontiers in immunology · 2018Review
- Sacral nerve stimulation enhances early intestinal mucosal repair following mucosal injury in a pig model.The Journal of physiology · 2016Article
- TNF-α-stimulated fibroblasts secrete lumican to promote fibrocyte differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Galectin-3 Binding Protein Secreted by Breast Cancer Cells Inhibits Monocyte-Derived Fibrocyte Differentiation.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Conserved Amblyomma americanum tick Serpin19, an inhibitor of blood clotting factors Xa and XIa, trypsin and plasmin, has anti-haemostatic functions.International journal for parasitology · 2015Article
- A brief exposure to tryptase or thrombin potentiates fibrocyte differentiation in the presence of serum or serum amyloid p.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- The long pentraxin PTX3 promotes fibrocyte differentiation.PloS one · 2015Article
- Trypsin, Tryptase, and Thrombin Polarize Macrophages towards a Pro-Fibrotic M2a Phenotype.PloS one · 2015Article
- Fibroblasts secrete Slit2 to inhibit fibrocyte differentiation and fibrosis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A blood meal-induced Ixodes scapularis tick saliva serpin inhibits trypsin and thrombin, and interferes with platelet aggregation and blood clotting.International journal for parasitology · 2014Article
- Peritoneal Dialysis Fluid and Some of Its Components Potentiate Fibrocyte Differentiation.Peritoneal dialysis international : journal of the International Society for Peritoneal DialysisArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Trypsin-containing topical treatments can be used to speed wound healing, although the mechanism of action is unknown. To help form granulation tissue and heal wounds, monocytes leave the circulation, enter the wound tissue, and differentiate into fibroblast-like cells called fibrocytes. We find that 20 to 200 ng/ml trypsin (concentrations similar to those used in wound dressings) potentiates the differentiation of human monocytes to fibrocytes in cell culture. Adding trypsin inhibitors increases the amount of trypsin needed to potentiate fibrocyte differentiation, suggesting that the potentiating effect is dependent on trypsin proteolytic activity. Proteases with other site specificities such as pepsin, endoprotease GluC, and chymotrypsin do not potentiate fibrocyte differentiation. This potentiation requires the presence of albumin in the culture medium, and tryptic fragments of human or bovine albumin also potentiate fibrocyte differentiation. These results suggest that topical trypsin speeds wound healing by generating tryptic fragments of albumin, which in turn potentiate fibrocyte differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.