ArticlePloS one2013
Prolonged mechanical ventilation alters the expression pattern of angio-neogenetic factors in a pre-clinical rat model.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Effects of elevated positive end-expiratory pressure on diaphragmatic blood flow and vascular resistance during mechanical ventilation.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Trial
- Intraoperative phrenic nerve stimulation to prevent diaphragm fiber weakness during thoracic surgery.PloS one · 2025Article
- Electrical stimulation mitigates muscle degradation shift in gene expressions during 12-h mechanical ventilation.Scientific reports · 2023Article
- Prolonged Mechanical Ventilation: Outcomes and Management.Journal of clinical medicine · 2022Review
- Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model.Intensive care medicine experimental · 2021Article
- Suppression of Hypoxia-Inducible Factor 1α by Low-Molecular-Weight Heparin Mitigates Ventilation-Induced Diaphragm Dysfunction in a Murine Endotoxemia Model.International journal of molecular sciences · 2021Article
- Low-Molecular-Weight Heparin Reduces Ventilation-Induced Lung Injury through Hypoxia Inducible Factor-1α in a Murine Endotoxemia Model.International journal of molecular sciences · 2020Article
- Skeletal myofiber VEGF deficiency leads to mitochondrial, structural, and contractile alterations in mouse diaphragm.Journal of applied physiology (Bethesda, Md. : 1985) · 2019Article
- Impaired diaphragm resistance vessel vasodilation with prolonged mechanical ventilation.Journal of applied physiology (Bethesda, Md. : 1985) · 2019Article
- Angiotensin-(1-7) exerts a protective action in a rat model of ventilator-induced diaphragmatic dysfunction.Intensive care medicine experimental · 2019Article
- Kinetics of ventilation-induced changes in diaphragmatic metabolism by bilateral phrenic pacing in a piglet model.Scientific reports · 2016Article
- Dysfunction of respiratory muscles in critically ill patients on the intensive care unit.Journal of cachexia, sarcopenia and muscle · 2016Review
- Expression of the Mas receptor is upregulated in skeletal muscle wasting.Histochemistry and cell biology · 2015Article
- [Ventilator-induced diaphragm dysfunction : clinically relevant problem].Der Anaesthesist · 2014Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveMechanical ventilation (MV) is a life saving intervention for patients with respiratory failure. Even after 6 hours of MV, diaphragm atrophy and dysfunction (collectively referred to as ventilator-induced diaphragmatic dysfunction, VIDD) occurs in concert with a blunted blood flow and oxygen delivery. The regulation of hypoxia sensitive factors (i.e. hypoxia inducible factor 1α, 2α (HIF-1α,-2α), vascular endothelial growth factor (VEGF)) and angio-neogenetic factors (angiopoietin 1-3, Ang) might contribute to reactive and compensatory alterations in diaphragm muscle.
methodsMale Wistar rats (n = 8) were ventilated for 24 hours or directly sacrificed (n = 8), diaphragm and mixed gastrocnemius muscle tissue was removed. Quantitative real time PCR and western blot analyses were performed to detect changes in angio-neogenetic factors and inflammatory markers. Tissues were stained using Isolectin (IB 4) to determine capillarity and calculate the capillary/fiber ratio.
resultsMV resulted in up-regulation of Ang 2 and HIF-1α mRNA in both diaphragm and gastrocnemius, while VEGF mRNA was down-regulated in both tissues. HIF-2α mRNA was reduced in both tissues, while GLUT 4 mRNA was increased in gastrocnemius and reduced in diaphragm samples. Protein levels of VEGF, HIF-1α, -2α and 4 did not change significantly. Additionally, inflammatory cytokine mRNA (Interleukin (IL)-6, IL-1β and TNF α) were elevated in diaphragm tissue.
conclusionThe results demonstrate that 24 hrs of MV and the associated limb disuse induce an up-regulation of angio-neogenetic factors that are connected to HIF-1α. Changes in HIF-1α expression may be due to several interactions occurring during MV.
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