ArticlePloS one2013
Immunohistochemical, ultrastructural and functional analysis of axonal regeneration through peripheral nerve grafts containing Schwann cells expressing BDNF, CNTF or NT3.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 68 citations in OpenAlex.
- Application strategies of autologous and decellularized nerve grafts: Structural and functional recovery.Neural regeneration research · 2026Article
- Successful polyethylene glycol fusion repair using stored viable peripheral nerve allografts in Sprague-Dawley and Lewis rats.Neural regeneration research · 2026Article
- SARM1 Inhibition Maintains Axonal Integrity After Rat Sciatic Nerve Transection and Repair.The Journal of hand surgery · 2025Article
- Engineering the Future of Restorative Clinical Peripheral Nerve Surgery.Advanced healthcare materials · 2025Review
- Creating Alternative Afferent Input to Facilitate the Regeneration of Injured Primary Afferent Neurons.Cureus · 2025Article
- The Role of Tissue Engineering and Three-Dimensional-Filled Conduits in Bridging Nerve Gaps: A Review of Recent Advancements.Journal of hand surgery global online · 2024Review
- Limited Nerve Regeneration across Acellular Nerve Allografts (ANAs) Coincides with Changes in Blood Vessel Morphology and the Development of a Pro-Inflammatory Microenvironment.International journal of molecular sciences · 2024Article
- Quantitative evaluation of rat sciatic nerve degeneration using high-frequency ultrasound.Scientific reports · 2023Article
- Inverted human umbilical artery as a 3D scaffold for sciatic nerve regeneration in rats.Cell and tissue banking · 2022Article
- Effects of amyloid precursor protein peptide APP96-110, alone or with human mesenchymal stromal cells, on recovery after spinal cord injury.Neural regeneration research · 2022Article
- Sortilin Modulates Schwann Cell Signaling and Remak Bundle Regeneration Following Nerve Injury.Frontiers in cellular neuroscience · 2022Article
- GFAP and desmin expression in lymphatic tissues leads to difficulties in distinguishing between glial and stromal cells.Scientific reports · 2021Article
- Article
- Evaluation of two methods to isolate Schwann cells from murine sciatic nerve.Journal of neuroscience methods · 2020Article
- Machine intelligence for nerve conduit design and production.Journal of biological engineering · 2020Review
- Claudin-15 overexpression inhibits proliferation and promotes apoptosis of Schwann cellsNeural regeneration research · 2020Article
- Novel Glial Cell Functions: Extensive Potency, Stem Cell-Like Properties, and Participation in Regeneration and Transdifferentiation.Frontiers in cell and developmental biology · 2020Review
- Fresh human amniotic membrane effectively promotes the repair of injured common peroneal nerve.Neural regeneration research · 2019Article
- DNMT1, a Novel Regulator Mediating mTORC1/mTORC2 Pathway-Induced NGF Expression in Schwann Cells.Neurochemical research · 2018Article
- An update-tissue engineered nerve grafts for the repair of peripheral nerve injuries.Neural regeneration research · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used morphological, immunohistochemical and functional assessments to determine the impact of genetically-modified peripheral nerve (PN) grafts on axonal regeneration after injury. Grafts were assembled from acellular nerve sheaths repopulated ex vivo with Schwann cells (SCs) modified to express brain-derived neurotrophic factor (BDNF), a secretable form of ciliary neurotrophic factor (CNTF), or neurotrophin-3 (NT3). Grafts were used to repair unilateral 1 cm defects in rat peroneal nerves and 10 weeks later outcomes were compared to normal nerves and various controls: autografts, acellular grafts and grafts with unmodified SCs. The number of regenerated βIII-Tubulin positive axons was similar in all grafts with the exception of CNTF, which contained the fewest immunostained axons. There were significantly lower fiber counts in acellular, untransduced SC and NT3 groups using a PanNF antibody, suggesting a paucity of large caliber axons. In addition, NT3 grafts contained the greatest number of sensory fibres, identified with either IB4 or CGRP markers. Examination of semi- and ultra-thin sections revealed heterogeneous graft morphologies, particularly in BDNF and NT3 grafts in which the fascicular organization was pronounced. Unmyelinated axons were loosely organized in numerous Remak bundles in NT3 grafts, while the BDNF graft group displayed the lowest ratio of umyelinated to myelinated axons. Gait analysis revealed that stance width was increased in rats with CNTF and NT3 grafts, and step length involving the injured left hindlimb was significantly greater in NT3 grafted rats, suggesting enhanced sensory sensitivity in these animals. In summary, the selective expression of BDNF, CNTF or NT3 by genetically modified SCs had differential effects on PN graft morphology, the number and type of regenerating axons, myelination, and locomotor function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.