ArticlePloS one2013
Identification and characterization of three novel lipases belonging to families II and V from Anaerovibrio lipolyticus 5ST.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 50 citations in OpenAlex.
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- Culture-independent discovery of a novel thermotolerant lipase and its producer from mesophilic anaerobic digestion sludge.Applied microbiology and biotechnology · 2025Article
- Potential of fermented herbal extracts to modulate digestion and gut microbiota during the weaner and fattening period on commercial pig farms.Frontiers in veterinary science · 2025Article
- Comparison of Fecal Microbiota and Metabolites Between Captive and Grazing Male Reindeer.Animals : an open access journal from MDPI · 2024Article
- Dynamic Changes in the Nutrient Digestibility, Rumen Fermentation, Serum Parameters of Perinatal Ewes and Their Relationship with Rumen Microbiota.Animals : an open access journal from MDPI · 2024Article
- Moringa leaf meal exerts growth benefits in small ruminants through modulating the gastrointestinal microbiome.Applied microbiology and biotechnology · 2024Article
- A lipase from Lacticaseibacillus rhamnosus IDCC 3201 with thermostability and pH resistance for use as a detergent additive.Applied microbiology and biotechnology · 2024Article
- Metagenomic sequencing identified microbial species in the rumen and cecum microbiome responsible for niacin treatment and related to intramuscular fat content in finishing cattle.Frontiers in microbiology · 2024Article
- Effect of Energy Provision Strategy on Rumen Fermentation Characteristics, Bacterial Diversity and Community Composition.Bioengineering (Basel, Switzerland) · 2023Article
- Dietary selection of metabolically distinct microorganisms drives hydrogen metabolism in ruminants.The ISME journal · 2022Article
- Dietary shifts and social interactions drive temporal fluctuations of the gut microbiome from wild redfronted lemurs.ISME communications · 2022Article
- Improving the catalytic efficiency and substrate affinity of a novel esterase from marine Klebsiella aerogenes by random and site-directed mutation.World journal of microbiology & biotechnology · 2021Article
- Metagenomic Analysis Reveals New Microbiota Related to Fiber Digestion in Pigs.Frontiers in microbiology · 2021Article
- Feeding Systems and Host Breeds Influence Ruminal Fermentation, Methane Production, Microbial Diversity and Metagenomic Gene Abundance.Frontiers in microbiology · 2021Article
- Advances in fatty acids nutrition in dairy cows: from gut to cells and effects on performance.Journal of animal science and biotechnology · 2020Review
- Different Sources of High Fat Diet Induces Marked Changes in Gut Microbiota of Nursery Pigs.Frontiers in microbiology · 2020Article
- Article
- Characterization and Immobilization of a Novel SGNH Family Esterase (International journal of molecular sciences · 2019Article
- Potential role of rumen microbiota in altering average daily gain and feed efficiency in meat goats fed simple and mixed pastures using bacterial tag-encoded FLX amplicon pyrosequencing1.Journal of animal science · 2019Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Following the isolation, cultivation and characterization of the rumen bacterium Anaerovibrio lipolyticus in the 1960s, it has been recognized as one of the major species involved in lipid hydrolysis in ruminant animals. However, there has been limited characterization of the lipases from the bacterium, despite the importance of understanding lipolysis and its impact on subsequent biohydrogenation of polyunsaturated fatty acids by rumen microbes. This study describes the draft genome of Anaerovibrio lipolytica 5ST, and the characterization of three lipolytic genes and their translated protein. The uncompleted draft genome was 2.83 Mbp and comprised of 2,673 coding sequences with a G+C content of 43.3%. Three putative lipase genes, alipA, alipB and alipC, encoding 492-, 438- and 248- amino acid peptides respectively, were identified using RAST. Phylogenetic analysis indicated that alipA and alipB clustered with the GDSL/SGNH family II, and alipC clustered with lipolytic enzymes from family V. Subsequent expression and purification of the enzymes showed that they were thermally unstable and had higher activities at neutral to alkaline pH. Substrate specificity assays indicated that the enzymes had higher hydrolytic activity against caprylate (C8), laurate (C12) and myristate (C14).
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