ArticlePloS one2013
No serological evidence of influenza A H1N1pdm09 virus infection as a contributing factor in childhood narcolepsy after Pandemrix vaccination campaign in Finland.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Influenza vaccination in children primed with MF59-adjuvanted or non-adjuvanted seasonal influenza vaccine.Human vaccines & immunotherapeutics · 2015Trial
- Patient experiences of narcolepsy and idiopathic hypersomnia in the Nordics: a patient journey map.Journal of sleep research · 2025Article
- The 2009 H1N1 pandemic, vaccine-associated narcolepsy, and the politics of risk and harm.Health (London, England : 1997) · 2022Article
- Narcolepsy and H1N1 influenza immunology a decade later: What have we learned?Frontiers in immunology · 2022Review
- A/H1N1 hemagglutinin antibodies show comparable affinity in vaccine-related Narcolepsy type 1 and control and are unlikely to contribute to pathogenesis.Scientific reports · 2021Article
- Review
- Detection of anti-NS1 antibodies after pandemic influenza exposure: Evaluation of a serological method for distinguishing H1N1pdm09 infected from vaccinated cases.Influenza and other respiratory viruses · 2020Article
- No evidence of autoimmunity to human OXJournal of translational autoimmunity · 2020Article
- Meeting report narcolepsy and pandemic influenza vaccination: What we know and what we need to know before the next pandemic? A report from the 2nd IABS meeting.Biologicals : journal of the International Association of Biological Standardization · 2019Article
- Narcolepsy Associated with Pandemrix Vaccine.Current neurology and neuroscience reports · 2018Review
- Autoimmunity in narcolepsy.Current opinion in pulmonary medicine · 2017Review
- Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases.PloS one · 2017Article
- Pandemrix™ and narcolepsy: A critical appraisal of the observational studies.Human vaccines & immunotherapeutics · 2016Review
- Clinical features of narcolepsy in children vaccinated with AS03 adjuvanted pandemic A/H1N1 2009 influenza vaccine in England.Developmental medicine and child neurology · 2014Article
- Narcolepsy patients have antibodies that stain distinct cell populations in rat brain and influence sleep patterns.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- History of narcolepsy at Stanford University.Immunologic research · 2014Review
- Risk of narcolepsy associated with inactivated adjuvanted (AS03) A/H1N1 (2009) pandemic influenza vaccine in Quebec.PloS one · 2014Article
- Article
- Narcolepsy and A(H1N1)pdm09 vaccination: shaping the research on the observed signal.Human vaccines & immunotherapeutics · 2014Article
Corrections and comments
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Authors and funding
16 authors at 10 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNarcolepsy cataplexy syndrome, characterised by excessive daytime sleepiness and cataplexy, is strongly associated with a genetic marker, human leukocyte antigen (HLA) DQB1*06:02. A sudden increase in the incidence of childhood narcolepsy was observed after vaccination with AS03-adjuvanted Pandemrix influenza vaccine in Finland at the beginning of 2010. Here, we analysed whether the coinciding influenza A H1N1pdm pandemic contributed, together with the Pandemrix vaccination, to the increased incidence of childhood narcolepsy in 2010. The analysis was based on the presence or absence of antibody response against non-structural protein 1 (NS1) from H1N1pdm09 virus, which was not a component of Pandemrix vaccine.
methodsNon-structural (NS) 1 proteins from recombinant influenza A/Udorn/72 (H3N2) and influenza A/Finland/554/09 (H1N1pdm09) viruses were purified and used in Western blot analysis to determine specific antibody responses in human sera. The sera were obtained from 45 patients who fell ill with narcolepsy after vaccination with AS03-adjuvanted Pandemrix at the end of 2009, and from controls.
findingsBased on quantitative Western blot analysis, only two of the 45 (4.4%) Pandemrix-vaccinated narcoleptic patients showed specific antibody response against the NS1 protein from the H1N1pdm09 virus, indicating past infection with the H1N1pdm09 virus. Instead, paired serum samples from patients, who suffered from a laboratory confirmed H1N1pdm09 infection, showed high levels or diagnostic rises (96%) in H1N1pdm virus NS1-specific antibodies and very high cross-reactivity to H3N2 subtype influenza A virus NS1 protein.
conclusionBased on our findings, it is unlikely that H1N1pdm09 virus infection contributed to a sudden increase in the incidence of childhood narcolepsy observed in Finland in 2010 after AS03-adjuvanted Pandemrix vaccination.
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