ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2013
High-throughput sequencing of a single chromosome: a moth W chromosome.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 55 citations in OpenAlex.
- Article
- Molecular mechanisms of sex determination in Lepidoptera: current status and perspectives.Insect science · 2026Review
- The complete mitochondrial genome and phylogenetic position ofMitochondrial DNA. Part B, Resources · 2026Article
- A W chromosome-derived feminizing piRNA in pyralid moths demonstrates convergent evolution for primary sex determination signals in Lepidoptera.BMC biology · 2025Article
- Article
- Article
- The complete mitochondrial genome ofMitochondrial DNA. Part B, Resources · 2024Article
- The complete mitochondrial genome analysis ofMitochondrial DNA. Part B, Resources · 2024Article
- Chromosome-Level Assemblies of the Pieris mannii Butterfly Genome Suggest Z-Origin and Rapid Evolution of the W Chromosome.Genome biology and evolution · 2023Article
- Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes rhomboidaria (Lepidoptera: Geometridae).Scientific reports · 2023Article
- Article
- Recovering individual haplotypes and a contiguous genome assembly from pooled long-read sequencing of the diamondback moth (Lepidoptera: Plutellidae).G3 (Bethesda, Md.) · 2022Article
- Nine Mitochondrial Genomes of the Pyraloidea and Their Phylogenetic Implications (Lepidoptera).Insects · 2021Article
- Article
- Article
- The First Mitogenomes of the Subfamily Odontiinae (Lepidoptera, Crambidae) and Phylogenetic Analysis of Pyraloidea.Insects · 2021Article
- Genomic Tackling of Human Satellite DNA: Breaking Barriers through Time.International journal of molecular sciences · 2021Review
- Bridging the Gap between Vertebrate Cytogenetics and Genomics with Single-Chromosome Sequencing (ChromSeq).Genes · 2021Review
- The Role of Satellite DNAs in Genome Architecture and Sex Chromosome Evolution in Crambidae Moths.Frontiers in genetics · 2021Article
- Characterization of the First W-Specific Protein-Coding Gene for Sex Identification inFrontiers in genetics · 2020Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Y and W chromosomes have mostly been excluded from whole genome sequencing projects. Due to the high amount of repetitive sequences they are 'difficult' to assemble and therefore need special treatment in the form of, e.g. adapted assembly programs, a range of different libraries, and accurate maps, if possible. A minimum requirement for these approaches is pure template DNA. We therefore microdissected the W chromatin of highly polyploid cells from the flour moth, Ephestia kuehniella, and used Roche/454 and Sanger sequencing to generate 72.6 Mbp of DNA sequence. Nominal coverage was 4.3× of the 16.7 Mbp of W chromosomal DNA. We used these data to assess the genetic content of the W chromosome. This approach allowed us to determine constituent families of transposable elements, microsatellites, and recent insertion sites of mitochondrial DNA. However, no conventional protein-coding gene has yet been found. The sequence collection is a rich source for the definition of W-specific PCR markers and the reconstruction of W chromosome loci, as a step towards full reconstruction of the chromosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.