Evidence map›Paper›PMID 23949429›Full record

ArticleEpigenetics2013

DNA methylation analysis of murine hematopoietic side population cells during aging.

Oluwatosin Taiwo, Gareth A Wilson, Warren Emmett, Tiffany Morris, Dominique Bonnet, Eugene Schuster, Tomas Adejumo, Stephan Beck, Daniel J Pearce

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epigenetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. DNA hypermethylation ofEpigenetics · 2022
    Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Current perspectives on the cellular and molecular features of epigenetic ageing.Experimental biology and medicine (Maywood, N.J.) · 2020
    Review
  11. Review
  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Review
  18. Review
  19. Review
  20. Class II major histocompatibility complex mutant mice to study the germ-line bias of T-cell antigen receptors.Proceedings of the National Academy of Sciences of the United States of America · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Oluwatosin TaiwoMedical Genomics; UCL Cancer Institute; University College London; London, UK; UCL Institute of Healthy Ageing; University College London; London, UK.
Gareth A WilsonMedical Genomics; UCL Cancer Institute; University College London; London, UK.
Warren EmmettUCL Genetics Institute; University College London; London, UK.
Tiffany MorrisMedical Genomics; UCL Cancer Institute; University College London; London, UK.
Dominique BonnetCancer Research UK; London Research Institute; Lincoln's Inn Fields; London, UK.
Eugene SchusterUCL Institute of Healthy Ageing; University College London; London, UK.
Tomas AdejumoScientific Support Services; Flow Facility; University College London; London, UK.
Stephan BeckMedical Genomics; UCL Cancer Institute; University College London; London, UK; These senior authors contributed equally to this work.
Daniel J PearceUCL Institute of Healthy Ageing; University College London; London, UK; These senior authors contributed equally to this work.

Funding

Medical Research Council G0800339Wellcome Trust 084071
6 · The paper itself

Abstract

Stem cells have been found in most tissues/organs. These somatic stem cells produce replacements for lost and damaged cells, and it is not completely understood how this regenerative capacity becomes diminished during aging. To study the possible involvement of epigenetic changes in somatic stem cell aging, we used murine hematopoiesis as a model system. Hematopoietic stem cells (HSCs) were enriched for via Hoechst exclusion activity (SP-HSC) from young, medium-aged and old mice and subjected to comprehensive, global methylome (MeDIP-seq) analysis. With age, we observed a global loss of DNA methylation of approximately 5%, but an increase in methylation at some CpG islands. Just over 100 significant (FDR<0.2) aging-specific differentially methylated regions (aDMRs) were identified, which are surprisingly few considering the profound age-based changes that occur in HSC biology. Interestingly, the polycomb repressive complex -2 (PCRC2) target genes Kiss1r, Nav2 and Hsf4 were hypermethylated with age. The promoter for the Sdpr gene was determined to be progressively hypomethylated with age. This occurred concurrently with an increase in gene expression with age. To explore this relationship further, we cultured isolated SP-HSC in the presence of 5-aza-deoxycytdine and demonstrated a negative correlation between Sdpr promoter methylation and gene expression. We report that DNA methylation patterns are well preserved during hematopoietic stem cell aging, confirm that PCRC2 targets are increasingly methylated with age, and suggest that SDPR expression changes with age in HSCs may be regulated via age-based alterations in DNA methylation.

Indexed as

AgingAnimalsCarrier ProteinsDNA MethylationFemaleHematopoiesisHematopoietic Stem CellsHumansMiceMice, Inbred C57BLPhosphate-Binding ProteinsPolycomb Repressive Complex 2Promoter Regions, GeneticSide-Population CellsCarrier ProteinsCAVIN2 protein, humanPhosphate-Binding ProteinsPolycomb Repressive Complex 2agingDNA methylationepigeneticshematopoietic stem cellsHsf4Kiss1rmethylomemethylomicsNano-MeDIP-seqNav2Sdpr polycomb repressive complex -2 (PCRC2)

Identifiers

PMID23949429
PMCPMC3891692

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.