ArticlePlant molecular biology2014
Hv-CBF2A overexpression in barley accelerates COR gene transcript accumulation and acquisition of freezing tolerance during cold acclimation.
Article in Plant molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 65 citations in OpenAlex.
- Vernalization of Winter Crops Increases Photosynthetic Energy Conversion Efficiency and Seed Yield.Plants (Basel, Switzerland) · 2025Review
- A barley pan-transcriptome reveals layers of genotype-dependent transcriptional complexity.Nature genetics · 2025Article
- Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions.Applied biochemistry and biotechnology · 2024Review
- Maize miRNAs and their putative target genes involved in chilling stress response in 5-day old seedlings.BMC genomics · 2024Article
- TheCells · 2023Review
- Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance.Journal of Zhejiang University. Science. B · 2023Review
- Cold acclimation alleviates cold stress-induced PSII inhibition and oxidative damage in tobacco leaves.Plant signaling & behavior · 2022Article
- Comprehensive analysis of cucumber C-repeat/dehydration-responsive element binding factor family genes and their potential roles in cold tolerance of cucumber.BMC plant biology · 2022Article
- Full-Length Transcriptome Sequencing Reveals the Impact of Cold Stress on Alternative Splicing in Quinoa.International journal of molecular sciences · 2022Article
- Comparative transcriptomic analyses of citrus cold-resistant vs. sensitive rootstocks might suggest a relevant role of ABA signaling in triggering cold scion adaption.BMC plant biology · 2022Article
- Phylotranscriptomics Resolves the Phylogeny of Pooideae and Uncovers Factors for Their Adaptive Evolution.Molecular biology and evolution · 2022Article
- Extensive allele mining discovers novel genetic diversity in the loci controlling frost tolerance in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Light Quality Modulates Plant Cold Response and Freezing Tolerance.Frontiers in plant science · 2022Article
- The Breeding of Winter-Hardy Malting Barley.Plants (Basel, Switzerland) · 2021Review
- Transcriptional memories mediate the plasticity of cold stress responses to enable morphological acclimation in Brachypodium distachyon.The New phytologist · 2021Article
- Identification of the Genetic Basis of Response to De-Acclimation in Winter Barley.International journal of molecular sciences · 2021Article
- Temperature and Light-Quality-Dependent Regulation of Freezing Tolerance in Barley.Plants (Basel, Switzerland) · 2020Article
- Engineering Multiple Abiotic Stress Tolerance in Canola,Frontiers in plant science · 2020Review
- Full-length transcriptome sequencing reveals the low-temperature-tolerance mechanism of Medicago falcata roots.BMC plant biology · 2019Article
- Cold acclimation by the CBF-COR pathway in a changing climate: Lessons from Arabidopsis thaliana.Plant cell reports · 2019Review
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
C-Repeat Binding Factors (CBFs) are DNA-binding transcriptional activators of gene pathways imparting freezing tolerance. Poaceae contain three CBF subfamilies, two of which, HvCBF3/CBFIII and HvCBF4/CBFIV, are unique to this taxon. To gain mechanistic insight into HvCBF4/CBFIV CBFs we overexpressed Hv-CBF2A in spring barley (Hordeum vulgare) cultivar 'Golden Promise'. The Hv-CBF2A overexpressing lines exhibited stunted growth, poor yield, and greater freezing tolerance compared to non-transformed 'Golden Promise'. Differences in freezing tolerance were apparent only upon cold acclimation. During cold acclimation freezing tolerance of the Hv-CBF2A overexpressing lines increased more rapidly than that of 'Golden Promise' and paralleled the freezing tolerance of the winter hardy barley 'Dicktoo'. Transcript levels of candidate CBF target genes, COR14B and DHN5 were increased in the overexpressor lines at warm temperatures, and at cold temperatures they accumulated to much higher levels in the Hv-CBF2A overexpressors than in 'Golden Promise'. Hv-CBF2A overexpression also increased transcript levels of other CBF genes at FROST RESISTANCE-H2-H2 (FR-H2) possessing CRT/DRE sites in their upstream regions, the most notable of which was CBF12. CBF12 transcript levels exhibited a relatively constant incremental increase above levels in 'Golden Promise' both at warm and cold. These data indicate that Hv-CBF2A activates target genes at warm temperatures and that transcript accumulation for some of these targets is greatly enhanced by cold temperatures.
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