ArticleMarine biotechnology (New York, N.Y.)2013
Characterisation of an L-haloacid dehalogenase from the marine psychrophile Psychromonas ingrahamii with potential industrial application.
Article in Marine biotechnology (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Antibacterial Activities and Life Cycle Stages ofMarine drugs · 2023Article
- Cold-Active Enzymes and Their Potential Industrial Applications-A Review.Molecules (Basel, Switzerland) · 2022Review
- Cold-Active β-Galactosidases: Insight into Cold Adaption Mechanisms and Biotechnological Exploitation.Marine drugs · 2021Review
- Functional Characterization of a L-2-Haloacid Dehalogenase FromFrontiers in microbiology · 2021Article
- Expression, Characterization and Its Deinking Potential of a Thermostable Xylanase FromFrontiers in bioengineering and biotechnology · 2021Article
- Mini Review: Advances in 2-Haloacid Dehalogenases.Frontiers in microbiology · 2021Review
- Deciphering the Structural Basis of High Thermostability of Dehalogenase from Psychrophilic BacteriumMicroorganisms · 2019Article
- Temperature adaptation of DNA ligases from psychrophilic organisms.Extremophiles : life under extreme conditions · 2019Article
- Applications of Marine-Derived Microorganisms and Their Enzymes in Biocatalysis and Biotransformation, the Underexplored Potentials.Frontiers in microbiology · 2019Review
- Marine-Derived Biocatalysts: Importance, Accessing, and Application in Aromatic Pollutant Bioremediation.Frontiers in microbiology · 2017Review
- Halophiles: biology, adaptation, and their role in decontamination of hypersaline environments.World journal of microbiology & biotechnology · 2016Review
- Integrated (Meta) Genomic and Synthetic Biology Approaches to Develop New Biocatalysts.Marine drugs · 2016Review
- Enzymes from Extreme Environments and Their Industrial Applications.Frontiers in bioengineering and biotechnology · 2015Review
- Emerging strategies and integrated systems microbiology technologies for biodiscovery of marine bioactive compounds.Marine drugs · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The recombinant L-haloacid dehalogenase from the marine bacterium Psychromonas ingrahamii has been cloned and over-expressed in Escherichia coli. It shows activity towards monobromoacetic (100 %), monochloroacetic acid (62 %), S-chloropropionic acid (42 %), S-bromopropionic acid (31 %), dichloroacetic acid (28 %) and 2-chlorobutyric acid (10 %), respectively. The L-haloacid dehalogenase has highest activity towards substrates with shorter carbon chain lengths (≤ C3), without preference towards a chlorine or bromine at the α-carbon position. Despite being isolated from a psychrophilic bacterium, the enzyme has mesophilic properties with an optimal temperature for activity of 45 °C. It retains above 70 % of its activity after being incubated at 65 °C for 90 min before being assayed at 25 °C. The enzyme is relatively stable in organic solvents as demonstrated by activity and thermal shift analysis. The V max and K m were calculated to be 0.6 μM min(-1) mg(-1) and 1.36 mM with monobromoacetic acid, respectively. This solvent-resistant and stable L-haloacid dehalogenase from P. ingrahamii has potential to be used as a biocatalyst in industrial processes.
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