ArticleBreast cancer research : BCR2013
Evidence for a multipotent mammary progenitor with pregnancy-specific activity.
Article in Breast cancer research : BCR, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- They will be famous: Multipotent stem cells in breast milk.World journal of clinical pediatrics · 2025Review
- Single-cell and spatial analyses reveal a tradeoff between murine mammary proliferation and lineage programs associated with endocrine cues.Cell reports · 2023Article
- Influence of Simulated Microgravity on Mammary Epithelial Cells Grown as 2D and 3D Cultures.International journal of molecular sciences · 2023Article
- Pregnancy reprograms the epigenome of mammary epithelial cells and blocks the development of premalignant lesions.Nature communications · 2020Article
- Long-label-retaining mammary epithelial cells are created early in ductal development and distributed throughout the branching ducts.Mechanisms of development · 2019Article
- Unraveling Heterogeneity in Epithelial Cell Fates of the Mammary Gland and Breast Cancer.Cancers · 2019Review
- Regulatory T Cells Control the Switch FromFrontiers in immunology · 2019Article
- Does the Mouse Mammary Gland Arise from Unipotent or Multipotent Mammary Stem/Progenitor Cells?Journal of mammary gland biology and neoplasia · 2018Review
- Long-lived unipotent Blimp1-positive luminal stem cells drive mammary gland organogenesis throughout adult life.Nature communications · 2017Article
- Lgr6 labels a rare population of mammary gland progenitor cells that are able to originate luminal mammary tumours.Nature cell biology · 2016Article
- Cell Fate Decisions During Breast Cancer Development.Journal of developmental biology · 2016Article
- Form and function: how estrogen and progesterone regulate the mammary epithelial hierarchy.Journal of mammary gland biology and neoplasia · 2015Review
- Mammary lineage tracing: the coming of age.Cellular and molecular life sciences : CMLS · 2015Review
- The mammary cellular hierarchy and breast cancer.Cellular and molecular life sciences : CMLS · 2014Review
- IGF1R inhibition in mammary epithelia promotes canonical Wnt signaling and Wnt1-driven tumors.Cancer research · 2014Article
- Mammary stem cells and the differentiation hierarchy: current status and perspectives.Genes & development · 2014Review
- The dynamics of murine mammary stem/progenitor cells.Frontiers in biology · 2014Article
- New insights into lineage restriction of mammary gland epithelium using parity-identified mammary epithelial cells.Breast cancer research : BCR · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
introductionThe mouse mammary gland provides a powerful model system for studying processes involved in epithelial tissue development. Although markers that enrich for mammary stem cells and progenitors have been identified, our understanding of the mammary developmental hierarchy remains incomplete.
methodsWe used the MMTV promoter linked to the reverse tetracycline transactivator to induce H2BGFP expression in the mouse mammary gland. Mammary epithelial cells (MECs) from virgin mice were sorted by flow cytometry for expression of the mammary stem cell/progenitor markers CD24 and CD29, and H2BGFP. Sorted populations were analyzed for in vivo repopulation ability, expression of mammary lineage markers, and differential gene expression.
resultsThe reconstituting activity of CD24⁺/CD29⁺ cells in cleared fat pad transplantation assays was not distinguished in GFP⁺ compared to GFP⁻ subpopulations. However, within the CD24⁺/CD29(lo) luminal progenitor-enriched population, H2BGFP⁺, but not H2BGFP⁻, MECs formed mammary structures in transplantation assays; moreover, this activity was dramatically enhanced in pregnant recipients. These outgrowths contained luminal and myoepithelial mammary lineages and produced milk, but lacked the capacity for serial transplantation. Transcriptional microarray analysis revealed that H2BGFP⁺/CD24⁺/CD29(lo) MECs are distinct from H2BGFP⁻/CD24⁺/CD29(lo) MECs and enriched for gene expression signatures with both the stem cell (CD24⁺/CD29⁺) and luminal progenitor (CD24⁺/CD29(lo)/CD61⁺) compartments.
conclusionsWe have identified a population of MECs containing pregnancy-activated multipotent progenitors that are present in the virgin mammary gland and contribute to the expansion of the mammary gland during pregnancy.
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Registered trials
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