ArticleJournal of clinical microbiology2014
Assessment of new culture method for detection of Borrelia species from serum of lyme disease patients.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Accuracy of Diagnostic Tests for Lyme Disease in Humans, A Systematic Review and Meta-Analysis of North American Research.PloS one · 2016Pooled it
- Persistent Symptoms After Treatment of Lyme Disease.Infectious disease clinics of North America · 2022Review
- Laboratory Diagnosis of Lyme Borreliosis.Clinical microbiology reviews · 2021Review
- Spatial and Temporal Comparison of Perceived Risks and Confirmed Cases of Lyme Disease: An Exploratory Study of Google Trends.Frontiers in public health · 2020Article
- Studies that report unexpected positive blood cultures for Lyme borrelia - are they valid?Diagnostic microbiology and infectious disease · 2017Review
- Accuracy of Diagnosis of Human Granulocytic Anaplasmosis in China.Emerging infectious diseases · 2016Article
- Current Guidelines, Common Clinical Pitfalls, and Future Directions for Laboratory Diagnosis of Lyme Disease, United States.Emerging infectious diseases · 2016Review
- Review
- Chronic Lyme disease.Infectious disease clinics of North America · 2015Review
- Laboratory diagnosis of Lyme disease: advances and challenges.Infectious disease clinics of North America · 2015Review
- Update on persistent symptoms associated with Lyme disease.Current opinion in pediatrics · 2015Review
- Repeat or persistent Lyme disease: persistence, recrudescence or reinfection with Borrelia Burgdorferi?F1000prime reports · 2015Review
- Laboratory diagnostics for Lyme disease.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2014Article
- Reply to "No evidence for contamination of Borrelia blood cultures: a review of facts".Journal of clinical microbiology · 2014Article
- No evidence for contamination of Borrelia blood cultures: a review of facts.Journal of clinical microbiology · 2014Article
- Concerns regarding a new culture method for Borrelia burgdorferi not approved for the diagnosis of Lyme disease.MMWR. Morbidity and mortality weekly report · 2014Article
Corrections and comments
- Commented on by
- Commented on by
- Erratum issued
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel method of culturing spirochetes from the serum of U.S. Lyme disease patients was recently reported by Sapi and colleagues to have 94% sensitivity and 100% specificity for Borrelia species as assessed by microscopy and DNA sequence analysis of the pyrG gene (E. Sapi, N. Pabbati, A. Datar, E. M. Davies, A. Rattelle, and B. A. Kuo, Int. J. Med. Sci. 10:362-376, 2013). The majority of the spirochetes described were related by pyrG sequences to species of Borrelia previously undetected in North American patients without a reported history of travel to Europe or Asia. To better understand these unexpected findings, we determined pyrG sequences of the laboratory reference strains used by the investigators for method development and testing of culture medium. Eighty percent (41/51) of the reported patient-derived pyrG sequences were identical to one of the laboratory strains, and an additional 12% (6/51) differed by only a single nucleotide across a 603-bp region of the pyrG gene. Thus, false positivity due to laboratory contamination of patient samples cannot be ruled out, and further validation of the proposed novel culture method is required.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.