Evidence map›Paper›PMID 23946439›Full record

ArticleDevelopment (Cambridge, England)2013

Differential roles for 3-OSTs in the regulation of cilia length and motility.

Judith M Neugebauer, Adam B Cadwallader, Jeffrey D Amack, Brent W Bisgrove, H Joseph Yost

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 20 citations in OpenAlex.

  1. Transcriptional divergence of the zebrafishbioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Review
  4. Deciphering functional glycosaminoglycan motifs in development.Current opinion in structural biology · 2018
    Review
  5. Article
  6. Article
  7. Article
  8. Kupffer's vesicle size threshold for robust left-right patterning of the zebrafish embryo.Developmental dynamics : an official publication of the American Association of Anatomists · 2016
    Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Heparan sulfate 3-O-sulfation: a rare modification in search of a function.Matrix biology : journal of the International Society for Matrix Biology · 2014
    Review
  14. Demystifying heparan sulfate-protein interactions.Annual review of biochemistry · 2014
    Review
  15. Article
  16. Left-right asymmetry: lessons from Cancún.Development (Cambridge, England) · 2013
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Judith M NeugebauerDepartment of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
Adam B Cadwallader
Jeffrey D Amack
Brent W Bisgrove
H Joseph Yost
University of Utah · US

Funding

RESEARCH TRAINING IN HEMATOLOGYT32DK007115 · NIDDK · UNIVERSITY OF UTAH · PI LEIBOLD, ELIZABETH ANN, PRCHAL, JOSEF T · 1986 to 2020
$8.4M
Molecular Roles of Syndecans in DevelopmentR01HL075472 · NHLBI · UNIVERSITY OF UTAH · PI YOST, H. JOSEPH · 2004 to 2008
$1.8M
NHLBI NIH HHS HL075472NHLBI NIH HHS R01 HL075472NIDDK NIH HHS T32 DK007115
6 · The paper itself

Abstract

As cells integrate molecular signals from their environment, cell surface receptors require modified proteoglycans for the robust activation of signaling pathways. Heparan sulfate proteoglycans (HSPGs) have long unbranched chains of repetitive disaccharide units that can be sulfated at specific positions by heparan sulfate O-sulfotransferase (OST) families. Here, we show that two members of the 3-OST family are required in distinct signaling pathways to control left-right (LR) patterning through control of Kupffer's vesicle (KV) cilia length and motility. 3-OST-5 functions in the fibroblast growth factor pathway to control cilia length via the ciliogenic transcription factors FoxJ1a and Rfx2. By contrast, a second 3-OST family member, 3-OST-6, does not regulate cilia length, but regulates cilia motility via kinesin motor molecule (Kif3b) expression and cilia arm dynein assembly. Thus, two 3-OST family members cell-autonomously control LR patterning through distinct pathways that regulate KV fluid flow. We propose that individual 3-OST isozymes create distinct modified domains or 'glycocodes' on cell surface proteoglycans, which in turn regulate the response to diverse cell signaling pathways.

Indexed as

AnimalsAnimal StructuresBody PatterningCiliaDyneinsEmbryo, NonmammalianFibroblast Growth FactorsKinesinsModels, BiologicalMorpholinosMovementSignal TransductionSulfotransferasesTranscription FactorsZebrafishZebrafish ProteinsDyneinsFibroblast Growth FactorsKinesinsMorpholinosSulfotransferasesTranscription FactorsZebrafish Proteins3-OSTCiliaFGFFibroblast growth factorsFluid flowGlycocodeGut asymmetryHeart asymmetryHeparan sulfate proteoglycansHSPGLeft-right developmentOSTO-SulfotransferaseZebrafish

Identifiers

PMID23946439
PMCPMC3754482
OpenAlexW2169918166

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.