ArticleJournal of immunology (Baltimore, Md. : 1950)2013
The tetraspanin CD63 is required for efficient IgE-mediated mast cell degranulation and anaphylaxis.
Article in Journal of immunology (Baltimore, Md. : 1950), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 65 citations in OpenAlex.
- Correlation Analysis Between Increased CD63 Expression Detected by Eosinophil Activation Test and sIgE Levels.Scandinavian journal of immunology · 2026Article
- Examining the immunomodulatory role of nanoparticles on mast cell activation.Scientific reports · 2026Article
- Alpha-gal-specific human IgE promotes both alpha-gal-induced and antigen-independent allergic effector cell activation.The Journal of allergy and clinical immunology · 2026Article
- Esculetin mitigates mast cell-mediated allergic inflammation and anaphylaxis by suppression FcεRI signaling.Inflammopharmacology · 2026Article
- Mast Cells Are Not Essential for Pubertal Mammary Gland Branching.European journal of immunology · 2025Article
- Maternal supplementation with α-tocopherol inhibits the development of offspring food allergy, H1R signaling and ultimately anaphylaxis early in life.Journal of immunology (Baltimore, Md. : 1950) · 2025Article
- RNF149 negatively regulates LPS/TLR4 signal transduction by ubiquitination-mediated CD63 degradation.Heliyon · 2024Article
- Cytotoxicity assessment of exfoliated MoSNanoscale advances · 2024Article
- A two-step activation mechanism enables mast cells to differentiate their response between extracellular and invasive enterobacterial infection.Nature communications · 2024Article
- Depression proteomic profiling in adolescents with transcriptome analyses in independent cohorts.Frontiers in psychiatry · 2024Article
- Role of dendritic cell‑derived exosomes in allergic rhinitis (Review).International journal of molecular medicine · 2023Review
- Secretory and Membrane-Associated Biomarkers of Mast Cell Activation and Proliferation.International journal of molecular sciences · 2023Review
- Mast cell surfaceome characterization reveals CD98 heavy chain is critical for optimal cell function.The Journal of allergy and clinical immunology · 2022Article
- CD63 acts as a functional marker in maintaining hematopoietic stem cell quiescence through supporting TGFβ signaling in mice.Cell death and differentiation · 2022Article
- Tetraspanins in the regulation of mast cell function.Medical microbiology and immunology · 2020Review
- Rapid Isolation of Rare Isotype-Switched Hybridoma Variants: Application to the Generation of IgG2a and IgG2b MAb to CD63, a Late Endosome and Exosome Marker.Antibodies (Basel, Switzerland) · 2020Article
- Article
- Inside-Out Control of Fc-Receptors.Frontiers in immunology · 2019Review
- An optimized protocol for the generation and functional analysis of human mast cells from CD34Journal of immunological methods · 2017Article
- Dysivillosins A-D, Unusual Anti-allergic Meroterpenoids from the Marine Sponge Dysidea villosa.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Mast cell (MC) activation through the high-affinity IgE receptor FcεRI leads to the release of mediators involved in immediate-type allergic reactions. Although Abs against the tetraspanins CD63 and CD81 inhibit FcεRI-induced MC degranulation, the intrinsic role of these molecules in FcεRI-induced MC activation is unknown. In MCs, CD63 is expressed at the cell surface and in lysosomes (particularly secretory lysosomes that contain allergic mediators). In this study, we investigated the role of CD63 in MC using a CD63 knockout mouse model. CD63-deficiency did not affect in vivo MC numbers and tissue distribution. Bone marrow-derived MC developed normally in the absence of CD63 protein. However, CD63-deficient bone marrow-derived MC showed a significant decrease in FcεRI-mediated degranulation, but not PMA/ionomycin-induced degranulation, as shown by β-hexosaminidase release assays. The secretion of TNF-α, which is both released from granules and synthesized de novo upon MC activation, was also decreased. IL-6 secretion and production of the lipid mediator leukotriene C₄ were unaffected. There were no ultrastructural differences in granule content and morphology, late endosomal/lysosomal marker expression, FcεRI-induced global tyrosine phosphorylation, and Akt phosphorylation. Finally, local reconstitution in genetically MC-deficient Kit(w/w-v) mice was unaffected by the absence of CD63. However, the sites reconstituted with CD63-deficient MC developed significantly attenuated cutaneous anaphylactic reactions. These findings demonstrate that the absence of CD63 results in a significant decrease of MC degranulation, which translates into a reduction of acute allergic reactions in vivo, thus identifying CD63 as an important component of allergic inflammation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.