ArticleRNA (New York, N.Y.)2013
2'-Phosphate cyclase activity of RtcA: a potential rationale for the operon organization of RtcA with an RNA repair ligase RtcB in Escherichia coli and other bacterial taxa.
Article in RNA (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 46 citations in OpenAlex.
- Enzymology of the metazoan tRNA ligase complex: a lifetime in cycles.Cellular and molecular life sciences : CMLS · 2026Review
- Heterogeneity in responses to ribosome-targeting antibiotics mediated by bacterial RNA repair.Nature communications · 2025Article
- Genome-wide characterization of hypothiocyanite stress response inJournal of bacteriology · 2025Article
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- Prophage terminase with tRNase activity sensitizesScience (New York, N.Y.) · 2024Article
- Anaerobiosis, a neglected factor in phage-bacteria interactions.Applied and environmental microbiology · 2023Article
- Insights into the structure and function of the RNA ligase RtcB.Cellular and molecular life sciences : CMLS · 2023Review
- Transfer RNA halves are found as nicked tRNAs in cells: evidence that nicked tRNAs regulate expression of an RNA repair operon.RNA (New York, N.Y.) · 2023Article
- Strain-Specific Gifsy-1 Prophage Genes Are Determinants for Expression of the RNA Repair Operon during the SOS Response in Salmonella enterica Serovar Typhimurium.Journal of bacteriology · 2023Article
- Interfering with nucleotide excision by the coronavirus 3'-to-5' exoribonuclease.Nucleic acids research · 2023Article
- The RNA repair proteins RtcAB regulate transcription activator RtcR via its CRISPR-associated Rossmann fold domain.iScience · 2022Article
- Interfering with nucleotide excision by the coronavirus 3'-to-5' exoribonuclease.bioRxiv : the preprint server for biology · 2022Article
- The expanding field of non-canonical RNA capping: new enzymes and mechanisms.Royal Society open science · 2021Review
- An RNA Repair Operon Regulated by Damaged tRNAs.Cell reports · 2020Article
- Evolutionary and functional classification of the CARF domain superfamily, key sensors in prokaryotic antivirus defense.Nucleic acids research · 2020Article
- The Bacterial Ro60 Protein and Its Noncoding Y RNA Regulators.Annual review of microbiology · 2020Review
- Ro60 and Y RNAs: structure, functions, and roles in autoimmunity.Critical reviews in biochemistry and molecular biology · 2019Review
- The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonicalThe Journal of biological chemistry · 2018Article
- Structural Basis for tRNA Mimicry by a Bacterial Y RNA.Structure (London, England : 1993) · 2018Article
- Genotoxic, Metabolic, and Oxidative Stresses Regulate the RNA Repair Operon of Salmonella enterica Serovar Typhimurium.Journal of bacteriology · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
RNA terminal phosphate cyclase catalyzes the ATP-dependent conversion of a 3'-phosphate RNA end to a 2',3'-cyclic phosphate via covalent enzyme-(histidinyl-Nε)-AMP and RNA(3')pp(5')A intermediates. Here, we report that Escherichia coli RtcA (and its human homolog Rtc1) are capable of cyclizing a 2'-phosphate RNA end in high yield. The rate of 2'-phosphate cyclization by RtcA is five orders of magnitude slower than 3'-phosphate cyclization, notwithstanding that RtcA binds with similar affinity to RNA3'p and RNA2'p substrates. These findings expand the functional repertoire of RNA cyclase and suggest that phosphate geometry during adenylate transfer to RNA is a major factor in the kinetics of cyclization. RtcA is coregulated in an operon with an RNA ligase, RtcB, that splices RNA 5'-OH ends to either 3'-phosphate or 2',3'-cyclic phosphate ends. Our results suggest that RtcA might serve an end healing function in an RNA repair pathway, by converting RNA 2'-phosphates, which cannot be spliced by RtcB, to 2',3'-cyclic phosphates that can be sealed. The rtcBA operon is controlled by the σ(54) coactivator RtcR encoded by an adjacent gene. This operon arrangement is conserved in diverse bacterial taxa, many of which have also incorporated the RNA-binding protein Ro (which is implicated in RNA quality control under stress conditions) as a coregulated component of the operon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.