ArticleJournal of cell science2013
Ca²⁺ and calpain control membrane expansion during the rapid cell spreading of neutrophils.
Article in Journal of cell science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Neutrophil Mechanosignaling Promotes Integrin Engagement With Endothelial Cells and Motility Within Inflamed Vessels.Frontiers in immunology · 2018Trial
- Review
- Cyclic stretch enhances neutrophil extracellular trap formation.BMC biology · 2024Article
- Localisation of Intracellular Signals and Responses during Phagocytosis.International journal of molecular sciences · 2023Review
- Review
- Effects of functionally diverse calpain system on immune cells.Immunologic research · 2021Review
- Neutrophil signaling during myocardial infarction wound repair.Cellular signalling · 2021Review
- Neutrophil Cell Shape Change: Mechanism and Signalling during Cell Spreading and Phagocytosis.International journal of molecular sciences · 2019Review
- Mechanistic Understanding of Single-Cell Behavior is Essential for Transformative Advances in Biomedicine.The Yale journal of biology and medicine · 2018Review
- Extension of chemotactic pseudopods by nonadherent human neutrophils does not require or cause calcium bursts.Science signaling · 2018Article
- Complement C5a-Induced Changes in Neutrophil Morphology During Inflammation.Scandinavian journal of immunology · 2017Article
- Topographical interrogation of the living cell surface reveals its role in rapid cell shape changes during phagocytosis and spreading.Scientific reports · 2017Article
- Critical role of calpain in inflammation.Biomedical reports · 2016Article
- Membrane Tension Acts Through PLD2 and mTORC2 to Limit Actin Network Assembly During Neutrophil Migration.PLoS biology · 2016Article
- Serum amyloid P: a systemic regulator of the innate immune response.Journal of leukocyte biology · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Following adherence of neutrophils to the endothelium, neutrophils undergo a major morphological change that is a necessary prelude to their extravasation. We show here that this shape change is triggered by an elevation of cytosolic inositol (1,4,5)-trisphosphate (IP3), to provoke physiological Ca(2+) influx through a store-operated mechanism. This transition from a spherical to 'flattened' neutrophil morphology is rapid (∼100 seconds) and is accompanied by an apparent rapid expansion of the area of the plasma membrane. However, no new membrane is added into the plasma membrane. Pharmacological inhibition of calpain-activation, which is triggered by Ca(2+) influx during neutrophil spreading, prevents normal cell flattening. In calpain-suppressed cells, an aberrant form of cell spreading can occur where an uncoordinated and localised expansion of the plasma membrane is evident. These data show that rapid neutrophil spreading is triggered by Ca(2+) influx, which causes activation of calpain and release of furled plasma membrane to allow its apparent 'expansion'.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.