ArticleThe Journal of biological chemistry2013
Phospholipase B is activated in response to sterol removal and stimulates acrosome exocytosis in murine sperm.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Genome-wide association study in Japanese females identifies fifteen novel skin-related trait associations.Scientific reports · 2018Trial
- 1-L Transcription of SARS-CoV-2 Spike Protein S1 Subunit.International journal of molecular sciences · 2024Article
- Lipid remodeling in acrosome exocytosis: unraveling key players in the human sperm.Frontiers in cell and developmental biology · 2024Review
- A genetically targeted sensor reveals spatial and temporal dynamics of acrosomal calcium and sperm acrosome exocytosis.The Journal of biological chemistry · 2022Article
- Selective measurement of NAPE-PLD activity via a PLAJournal of lipid research · 2022Article
- Membrane Remodeling and Matrix Dispersal Intermediates During Mammalian Acrosomal Exocytosis.Frontiers in cell and developmental biology · 2021Article
- Theoretical Analysis of S, M and N Structural Proteins by the Protein-RNA Recognition Code Leads to Genes/proteins that Are Relevant to the SARS-CoV-2 Life Cycle and Pathogenesis.Frontiers in genetics · 2021Article
- Src family kinases-mediated negative regulation of sperm acrosome reaction in chickens (Gallus gallus domesticus).PloS one · 2020Article
- Timing of sperm capacitation varies reproducibly among men.Molecular reproduction and development · 2018Article
- Cysteine dioxygenase is essential for mouse sperm osmoadaptation and male fertility.The FEBS journal · 2018Article
- Validation of a laboratory-developed test of human sperm capacitation.Molecular reproduction and development · 2017Article
- Characterization of the functions and proteomes associated with membrane rafts in chicken sperm.PloS one · 2017Article
- Comparison of Membrane Characteristics between Freshly Ejaculated and Cryopreserved Sperm in the Chicken.The journal of poultry science · 2016Article
- Organization of Membrane Rafts in Chicken Sperm.The journal of poultry science · 2016Article
- Progesterone-induced Acrosome Exocytosis Requires Sequential Involvement of Calcium-independent Phospholipase A2β (iPLA2β) and Group X Secreted Phospholipase A2 (sPLA2).The Journal of biological chemistry · 2016Article
- Leveraging Comparative Genomics to Identify and Functionally Characterize Genes Associated with Sperm Phenotypes in Python bivittatus (Burmese Python).Genetics research international · 2016Article
- Ultrastructure and lipid composition of detergent-resistant membranes derived from mammalian sperm and two types of epithelial cells.Cell and tissue research · 2016Article
- Unresolved questions concerning mammalian sperm acrosomal exocytosis.Biology of reproduction · 2014Review
- Membrane rafts regulate phospholipase B activation in murine sperm.Communicative & integrative biology · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Despite a strict requirement for sterol removal for sperm to undergo acrosome exocytosis (AE), the mechanisms by which changes in membrane sterols are transduced into changes in sperm fertilization competence are poorly understood. We have previously shown in live murine sperm that the plasma membrane overlying the acrosome (APM) contains several types of microdomains known as membrane rafts. When characterizing the membrane raft-associated proteomes, we identified phospholipase B (PLB), a calcium-independent enzyme exhibiting multiple activities. Here, we show that sperm surface PLB is activated in response to sterol removal. Both biochemical activity assays and immunoblots of subcellular fractions of sperm incubated with the sterol acceptor 2-hydroxypropyl-β-cyclodextrin (2-OHCD) confirmed the release of an active PLB fragment. Specific protease inhibitors prevented PLB activation, revealing a mechanistic requirement for proteolytic cleavage. Competitive inhibitors of PLB reduced the ability of sperm both to undergo AE and to fertilize oocytes in vitro, suggesting an important role in fertilization. This was reinforced by our finding that incubation either with protein concentrate released from 2-OHCD-treated sperm or with recombinant PLB peptide corresponding to the catalytic domain was able to induce AE in the absence of other stimuli. Together, these results lead us to propose a novel mechanism by which sterol removal promotes membrane fusogenicity and AE, helping confer fertilization competence. Importantly, this mechanism provides a basis for the newly emerging model of AE in which membrane fusions occur during capacitation/transit through the cumulus, prior to any physical contact between the sperm and the oocyte's zona pellucida.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.