ArticleCalcified tissue international2013
Effects of pH on the production of phosphate and pyrophosphate by matrix vesicles' biomimetics.
Article in Calcified tissue international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Design and validation of a perfusion bioreactor for long-term human bone explant culture.JBMR plus · 2026Article
- Modulation of TNAP activity and apatite formation in biomimetic matrix vesicles studied byBiochimica et biophysica acta. Biomembranes · 2025Article
- Shedding Light on the Role of Na,K-ATPase as a Phosphatase during Matrix-Vesicle-Mediated Mineralization.International journal of molecular sciences · 2022Article
- Biochemical and clinical manifestations in adults with hypophosphatasia: a national cross-sectional study.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2022Article
- Mineralization Profile of Annexin A6-Harbouring Proteoliposomes: Shedding Light on the Role of Annexin A6 on Matrix Vesicle-Mediated Mineralization.International journal of molecular sciences · 2022Article
- Axial mechanical loading toBone reports · 2021Article
- Time-dependently Appeared Microenvironmental Changes and Mechanism after Cartilage or Joint Damage and the Influences on Cartilage Regeneration.Organogenesis · 2021Review
- TNAP as a New Player in Chronic Inflammatory Conditions and Metabolism.International journal of molecular sciences · 2021Review
- Magnesium ions regulate mesenchymal stem cells population and osteogenic differentiation: A fuzzy agent-based modeling approach.Computational and structural biotechnology journal · 2021Article
- Tissue-Nonspecific Alkaline Phosphatase-A Gatekeeper of Physiological Conditions in Health and a Modulator of Biological Environments in Disease.Biomolecules · 2020Review
- Lipid composition modulates ATP hydrolysis and calcium phosphate mineral propagation by TNAP-harboring proteoliposomes.Archives of biochemistry and biophysics · 2020Article
- Preclinical safety and efficacy of 24R,25-dihydroxyvitamin DJournal of orthopaedic translation · 2020Article
- Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators.Journal of bone and mineral metabolism · 2019Article
- Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?Archives of biochemistry and biophysics · 2019Article
- Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models.Biochimica et biophysica acta. General subjects · 2018Review
- Biophysical aspects of biomineralization.Biophysical reviews · 2017Review
- Unilateral anterior crossbite induces aberrant mineral deposition in degenerative temporomandibular cartilage in rats.Osteoarthritis and cartilage · 2016Article
- Articular cartilage vesicles and calcium crystal deposition diseases.Current opinion in rheumatology · 2016Review
- Proteoliposomes with the ability to transport Ca(2+) into the vesicles and hydrolyze phosphosubstrates on their surface.Archives of biochemistry and biophysics · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
During endochondral bone formation, chondrocytes and osteoblasts synthesize and mineralize the extracellular matrix through a process that initiates within matrix vesicles (MVs) and ends with bone mineral propagation onto the collagenous scaffold. pH gradients have been identified in the growth plate of long bones, but how pH changes affect the initiation of skeletal mineralization is not known. Tissue-nonspecific alkaline phosphatase (TNAP) degrades extracellular inorganic pyrophosphate (PPi), a mineralization inhibitor produced by ectonucleotide pyrophosphatase/phosphodiesterase-1 (NPP1), while contributing Pi from ATP to initiate mineralization. TNAP and NPP1, alone or combined, were reconstituted in dipalmitoylphosphatidylcholine liposomes to mimic the microenvironment of MVs. The hydrolysis of ATP, ADP, AMP, and PPi was studied at pH 8 and 9 and compared to the data determined at pH 7.4. While catalytic efficiencies in general were higher at alkaline pH, PPi hydrolysis was maximal at pH 8 and indicated a preferential utilization of PPi over ATP at pH 8 versus 9. In addition, all proteoliposomes induced mineral formation when incubated in a synthetic cartilage lymph containing 1 mM ATP as substrate and amorphous calcium phosphate or calcium-phosphate-phosphatidylserine complexes as nucleators. Propagation of mineralization was significantly more efficient at pH 7.5 and 8 than at pH 9. Since a slight pH elevation from 7.4 to 8 promotes considerably more hydrolysis of ATP, ADP, and AMP primarily by TNAP, this small pH change facilitates mineralization, especially via upregulated PPi hydrolysis by both NPP1 and TNAP, further elevating the Pi/PPi ratio, thus enhancing bone mineralization.
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Registered trials
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