ArticleBMC genomics2013
Comparative genomic and transcriptome analyses of pathotypes of Xanthomonas citri subsp. citri provide insights into mechanisms of bacterial virulence and host range.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed.
- Improving a Tn7-based luciferase reporter system for promoter activity studies.Microbiology (Reading, England) · 2026Article
- Article
- Greater than the sum of their parts: an overview of the AvrRps4 effector family.Frontiers in plant science · 2024Review
- Phylogenomic analysis of 343 Xanthomonas citri pv. citri strains unravels introduction history and dispersal paths.PLoS pathogens · 2023Article
- Herbarium specimen sequencing allows precise dating of Xanthomonas citri pv. citri diversification history.Nature communications · 2023Article
- Complete genome sequence of an Israeli isolate ofFrontiers in plant science · 2023Article
- Citrus Canker Pathogen, Its Mechanism of Infection, Eradication, and Impacts.Plants (Basel, Switzerland) · 2022Review
- An Extracytoplasmic Function Sigma Factor Required for Full Virulence in Xanthomonas citri pv. citri.Journal of bacteriology · 2022Article
- Xanthomonas transcriptome inside cauliflower hydathodes reveals bacterial virulence strategies and physiological adaptations at early infection stages.Molecular plant pathology · 2022Article
- Comparative Genomic Analysis of the Lettuce Bacterial Leaf Spot Pathogen,Frontiers in microbiology · 2022Article
- The transcriptome landscapes of citrus leaf in different developmental stages.Plant molecular biology · 2021Article
- Phytochemical Screening and Evaluation of Antioxidant Properties and Antimicrobial Activity againstMolecules (Basel, Switzerland) · 2021Article
- Regulatory Mechanisms of the Resistance to Common Bacterial Blight Revealed by Transcriptomic Analysis in Common Bean (Frontiers in plant science · 2021Article
- Secrete or perish: The role of secretion systems inComputational and structural biotechnology journal · 2021Review
- Characterization of Basal Transcriptomes Identifies Potential Metabolic and Virulence-Associated Adaptations Among Diverse NontyphoidalFrontiers in microbiology · 2021Article
- Toxin-Antitoxin Gene Pairs Found in TnmBio · 2020Article
- Stringent response regulators (p)ppGpp and DksA positively regulate virulence and host adaptation of Xanthomonas citri.Molecular plant pathology · 2019Article
- Two forms of phosphomannomutase in gammaproteobacteria: The overlooked membrane-bound form of AlgC is required for twitching motility of Lysobacter enzymogenes.Environmental microbiology · 2019Article
- Deciphering how plant pathogenic bacteria disperse and meet: Molecular epidemiology ofEvolutionary applications · 2019Article
- Complete Genome Sequence of Sequevar 14MFrontiers in microbiology · 2019Article
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9 authors.
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Abstract
backgroundCitrus bacterial canker is a disease that has severe economic impact on citrus industries worldwide and is caused by a few species and pathotypes of Xanthomonas. X. citri subsp. citri strain 306 (XccA306) is a type A (Asiatic) strain with a wide host range, whereas its variant X. citri subsp. citri strain A(w)12879 (Xcaw12879, Wellington strain) is restricted to Mexican lime.
resultsTo characterize the mechanism for the differences in host range of XccA and Xcaw, the genome of Xcaw12879 that was completed recently was compared with XccA306 genome. Effectors xopAF and avrGf1 are present in Xcaw12879, but were absent in XccA306. AvrGf1 was shown previously for Xcaw to cause hypersensitive response in Duncan grapefruit. Mutation analysis of xopAF indicates that the gene contributes to Xcaw growth in Mexican lime but does not contribute to the limited host range of Xcaw. RNA-Seq analysis was conducted to compare the expression profiles of Xcaw12879 and XccA306 in Nutrient Broth (NB) medium and XVM2 medium, which induces hrp gene expression. Two hundred ninety two and 281 genes showed differential expression in XVM2 compared to in NB for XccA306 and Xcaw12879, respectively. Twenty-five type 3 secretion system genes were up-regulated in XVM2 for both XccA and Xcaw. Among the 4,370 common genes of Xcaw12879 compared to XccA306, 603 genes in NB and 450 genes in XVM2 conditions were differentially regulated. Xcaw12879 showed higher protease activity than XccA306 whereas Xcaw12879 showed lower pectate lyase activity in comparison to XccA306.
conclusionsComparative genomic analysis of XccA306 and Xcaw12879 identified strain specific genes. Our study indicated that AvrGf1 contributes to the host range limitation of Xcaw12879 whereas XopAF contributes to virulence. Transcriptome analyses of XccA306 and Xcaw12879 presented insights into the expression of the two closely related strains of X. citri subsp. citri. Virulence genes including genes encoding T3SS components and effectors are induced in XVM2 medium. Numerous genes with differential expression in Xcaw12879 and XccA306 were identified. This study provided the foundation to further characterize the mechanisms for virulence and host range of pathotypes of X. citri subsp. citri.
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