ArticleBMC developmental biology2013
Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development.
Article in BMC developmental biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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19 citing papers in PubMed, 53 citations in OpenAlex.
- Effect of Small Molecules on Blastocyst Development and Outgrowth Establishment of Bovine Haploid Parthenogenetic Embryos.Animals : an open access journal from MDPI · 2026Article
- Stage-sensitive potential of isolated rabbit ICM to differentiate into extraembryonic lineages†.Biology of reproduction · 2025Article
- Agouti-signaling protein (ASIP) improves bovine in vitro matured oocyte developmental competence and modulates lipid content.Journal of assisted reproduction and genetics · 2025Article
- Pre-Implantation Bovine Embryo Evaluation-From Optics to Omics and Beyond.Animals : an open access journal from MDPI · 2023Review
- Pluripotent Core in Bovine Embryos: A Review.Animals : an open access journal from MDPI · 2022Review
- Article
- Cell differentiation events in pre-implantation mouse and bovine embryos.Animal reproduction · 2021Review
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- Embryonic POU5F1 is Required for Expanded Bovine Blastocyst Formation.Scientific reports · 2018Article
- OCT4/POU5F1 is required for NANOG expression in bovine blastocysts.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Suppression of ERK signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo.Development (Cambridge, England) · 2017Article
- Article
- Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency.PloS one · 2017Article
- Establishment of bovine embryonic stem cells after knockdown of CDX2.Scientific reports · 2016Article
- Unique trophoblast stem cell- and pluripotency marker staining patterns depending on gestational age and placenta-associated pregnancy complications.Cell adhesion & migration · 2016Article
- Evidence supporting a role for SMAD2/3 in bovine early embryonic development: potential implications for embryotropic actions of follistatin.Biology of reproduction · 2015Article
- A mRNA landscape of bovine embryos after standard and MAPK-inhibited culture conditions: a comparative analysis.BMC genomics · 2015Article
- GATA Transcription Factors in Pregnancy.Medical journal of obstetrics and gynecology · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPreimplantation bovine development is emerging as an attractive experimental model, yet little is known about the mechanisms underlying trophoblast (TE)/inner cell mass (ICM) segregation in cattle. To gain an insight into these processes we have studied protein and mRNA distribution during the crucial stages of bovine development. Protein distribution of lineage specific markers OCT4, NANOG, CDX2 were analysed in 5-cell, 8-16 cell, morula and blastocyst stage embryos. ICM/TE mRNA levels were compared in hatched blastocysts and included: OCT4, NANOG, FN-1, KLF4, c-MYC, REX1, CDX2, KRT-18 and GATA6.
resultsAt the mRNA level the observed distribution patterns agree with the mouse model. CDX2 and OCT4 proteins were first detected in 5-cell stage embryos. NANOG appeared at the morula stage and was located in the cytoplasm forming characteristic rings around the nuclei. Changes in sub-cellular localisation of OCT4, NANOG and CDX2 were noted from the 8-16 cell onwards. CDX2 initially co-localised with OCT4, but at the blastocyst stage a clear lineage segregation could be observed. Interestingly, we have observed in a small proportion of embryos (2%) that CDX2 immunolabelling overlapped with mitotic chromosomes.
conclusionsCell fate specification in cattle become evident earlier than presently anticipated - around the time of bovine embryonic genome activation. There is an intriguing possibility that for proper lineage determination certain transcription factors (such as CDX2) may need to occupy specific regions of chromatin prior to its activation in the interphase nucleus. Our observation suggests a possible role of CDX2 in the process of epigenetic regulation of embryonic cell fate.
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