ArticlePloS one2013
Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- Integrated Genomic and Functional Analyses IdentifyInternational journal of molecular sciences · 2026Article
- Transcriptomic and metabolomic analysis reveals the role of Piriformospora indica in enhancing strawberry alkaline tolerance.BMC plant biology · 2026Article
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- Strawberry atlas:PeerJ · 2026Article
- The Role of Potassium and KUP/KT/HAK Transporters in Regulating Strawberry (Plants (Basel, Switzerland) · 2025Article
- Changes in soluble sugars and the expression of sugar transporter protein genes in strawberry crowns responding toPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- FaNPR3 Members of the NPR1-like Gene Family Negatively Modulate Strawberry Fruit Resistance againstPlants (Basel, Switzerland) · 2024Article
- The cacao gene atlas: a transcriptome developmental atlas reveals highly tissue-specific and dynamically-regulated gene networks in Theobroma cacao L.BMC plant biology · 2024Article
- Combined transcriptome and metabolome analysis revealed the molecular mechanisms of fruit skin coloration in pink strawberry.Frontiers in plant science · 2024Article
- Selection of Reference Genes inPlants (Basel, Switzerland) · 2023Article
- DNA methylation mediated by RdDM pathway and demethylation affects furanone accumulation through regulation ofHorticulture research · 2023Article
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- Evaluation of reference genes for transcript normalization inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- UnravelingPlants (Basel, Switzerland) · 2022Article
- A Comprehensive Study of the WRKY Transcription Factor Family in Strawberry.Plants (Basel, Switzerland) · 2022Article
- Genome-wide identification of ubiquitin proteasome subunits as superior reference genes for transcript normalization during receptacle development in strawberry cultivars.BMC genomics · 2021Article
- RNA-Seq and Gene Regulatory Network Analyses Uncover Candidate Genes in the Early Defense to Two HemibiotrophicFrontiers in genetics · 2021Article
- Evolution and roles of cytokinin genes in angiosperms 1: Do ancientHorticulture research · 2020Article
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- Solving the controversy of healthier organic fruit: Leaf wounding triggers distant gene expression response of polyphenol biosynthesis in strawberry fruit (Fragaria x ananassa).Scientific reports · 2019Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Strawberry (Fragaria spp) is an emerging model for the development of basic genomics and recombinant DNA studies among rosaceous crops. Functional genomic and molecular studies involve relative quantification of gene expression under experimental conditions of interest. Accuracy and reliability are dependent upon the choice of an optimal reference control transcript. There is no information available on validated endogenous reference genes for use in studies testing strawberry-pathogen interactions. Thirteen potential pre-selected strawberry reference genes were tested against different tissues, strawberry cultivars, biotic stresses, ripening and senescent conditions, and SA/JA treatments. Evaluation of reference candidate's suitability was analyzed by five different methodologies, and information was merged to identify best reference transcripts. A combination of all five methods was used for selective classification of reference genes. The resulting superior reference genes, FaRIB413, FaACTIN, FaEF1α and FaGAPDH2 are strongly recommended as control genes for relative quantification of gene expression in strawberry. This report constitutes the first systematic study to identify and validate optimal reference genes for accurate normalization of gene expression in strawberry plant defense response studies.
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