ArticlePloS one2013
Heterogeneity of astrocytes: from development to injury - single cell gene expression.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed, 138 citations in OpenAlex.
- Article
- Olfactory ensheathing cells from adult female rats are hybrid glia that promote neural repair.eLife · 2025Article
- The deletion of AQP4 and TRPV4 affects astrocyte swelling/volume recovery in response to ischemia-mimicking pathologies.Frontiers in cellular neuroscience · 2024Article
- Astrocyte-Neuron Interactions in Spinal Cord Injury.Advances in neurobiology · 2024Review
- How S100B crosses brain barriers and why it is considered a peripheral marker of brain injury.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- Single-Cell RNA-Sequencing in Astrocyte Development, Heterogeneity, and Disease.Cellular and molecular neurobiology · 2023Review
- Diverse but unique astrocytic phenotypes during embryonic stem cell differentiation, culturing and development.Communications biology · 2023Article
- Reactive astrogliosis in the era of single-cell transcriptomics.Frontiers in cellular neuroscience · 2023Review
- Modeling the heterogeneity of sodium and calcium homeostasis between cortical and hippocampal astrocytes and its impact on bioenergetics.Frontiers in cellular neuroscience · 2023Article
- Microfluidics for Neuronal Cell and Circuit Engineering.Chemical reviews · 2022Review
- Review
- Transient astrocytic mGluR5 expression drives synaptic plasticity and subsequent chronic pain in mice.The Journal of experimental medicine · 2022Article
- Cells of the Blood-Brain Barrier: An Overview of the Neurovascular Unit in Health and Disease.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Glial-Neuronal Interactions in Pathogenesis and Treatment of Spinal Cord Injury.International journal of molecular sciences · 2021Review
- Injury-induced ASCL1 expression orchestrates a transitory cell state required for repair of the neonatal cerebellum.Science advances · 2021Article
- Glial and myeloid heterogeneity in the brain tumour microenvironment.Nature reviews. Cancer · 2021Review
- Transient astrocyte-like NG2 glia subpopulation emerges solely following permanent brain ischemia.Glia · 2021Article
- Characterization of Astrocyte Morphology and Function Using a Fast and Reliable Tissue Clearing Technique.Current protocols · 2021Article
- Article
- The Characterization of a Subependymal Giant Astrocytoma-Like Cell Line from Murine Astrocyte with mTORC1 Hyperactivation.International journal of molecular sciences · 2021Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Astrocytes perform control and regulatory functions in the central nervous system; heterogeneity among them is still a matter of debate due to limited knowledge of their gene expression profiles and functional diversity. To unravel astrocyte heterogeneity during postnatal development and after focal cerebral ischemia, we employed single-cell gene expression profiling in acutely isolated cortical GFAP/EGFP-positive cells. Using a microfluidic qPCR platform, we profiled 47 genes encoding glial markers and ion channels/transporters/receptors participating in maintaining K(+) and glutamate homeostasis per cell. Self-organizing maps and principal component analyses revealed three subpopulations within 10-50 days of postnatal development (P10-P50). The first subpopulation, mainly immature glia from P10, was characterized by high transcriptional activity of all studied genes, including polydendrocytic markers. The second subpopulation (mostly from P20) was characterized by low gene transcript levels, while the third subpopulation encompassed mature astrocytes (mainly from P30, P50). Within 14 days after ischemia (D3, D7, D14), additional astrocytic subpopulations were identified: resting glia (mostly from P50 and D3), transcriptionally active early reactive glia (mainly from D7) and permanent reactive glia (solely from D14). Following focal cerebral ischemia, reactive astrocytes underwent pronounced changes in the expression of aquaporins, nonspecific cationic and potassium channels, glutamate receptors and reactive astrocyte markers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.