ArticlePloS one2013
Nitric oxide synthetic pathway in red blood cells is impaired in coronary artery disease.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- A 3D-printed, multi-modal microfluidic device for measuring nitric oxide and ATP release from flowing red blood cells.Analytical methods : advancing methods and applications · 2022Article
- Oxidative Stress and Arginine/Nitric Oxide Pathway in Red Blood Cells Derived from Patients with Prediabetes.Biomedicines · 2022Article
- Red Blood Cell and Endothelial eNOS Independently Regulate Circulating Nitric Oxide Metabolites and Blood Pressure.Circulation · 2021Article
- Conditioning medicine for ischemic and hemorrhagic stroke.Conditioning medicine · 2021Article
- The Mystery of Red Blood Cells Extracellular Vesicles in Sleep Apnea with Metabolic Dysfunction.International journal of molecular sciences · 2021Review
- Predialysis and Dialysis Therapies Differently Affect Nitric Oxide Synthetic Pathway in Red Blood Cells from Uremic Patients: Focus on Peritoneal Dialysis.International journal of molecular sciences · 2021Article
- Shear Stress and RBC-NOS Serine1177 Phosphorylation in Humans: A Dose Response.Life (Basel, Switzerland) · 2021Article
- Relationship Between Plasma Osteopontin and Arginine Pathway Metabolites in Patients With Overt Coronary Artery Disease.Frontiers in physiology · 2020Article
- Article
- Identification of a soluble guanylate cyclase in RBCs: preserved activity in patients with coronary artery disease.Redox biology · 2018Article
- Comparisons of Blood Parameters, Red Blood Cell Deformability and Circulating Nitric Oxide Between Males and Females Considering Hormonal Contraception: A Longitudinal Gender Study.Frontiers in physiology · 2018Article
- Red Blood Cell Function and Dysfunction: Redox Regulation, Nitric Oxide Metabolism, Anemia.Antioxidants & redox signaling · 2017Review
- Chitosan treatment abrogates hypercholesterolemia-induced erythrocyte's arginase activation.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2017Article
- Erythrocyte nanovesicles: Biogenesis, biological roles and therapeutic approach: Erythrocyte nanovesicles.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2017Review
- A Material Conferring Hemocompatibility.Scientific reports · 2016Article
- Nitric oxide synthetic pathway and cGMP levels are altered in red blood cells from end-stage renal disease patients.Molecular and cellular biochemistry · 2016Article
- Nitric oxide synthetic pathway in patients with microvascular angina and its relations with oxidative stress.Oxidative medicine and cellular longevity · 2014Article
- Levels of human erythrocyte membrane-bound and cytosolic glycohydrolases are associated with oxidative stress in erectile dysfunction patients.Disease markers · 2014Article
- Endothelial nitric oxide synthase in red blood cells: key to a new erythrocrine function?Redox biology · 2014Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAll the enzymatic factors/cofactors involved in nitric oxide (NO) metabolism have been recently found in red blood cells. Increased oxidative stress impairs NO bioavailability and has been described in plasma of coronary artery disease (CAD) patients. The aim of the study was to highlight a potential dysfunction of the metabolic profile of NO in red blood cells and in plasma from CAD patients compared with healthy controls.
methodsWe determined L-arginine/NO pathway by liquid-chromatography tandem mass spectrometry and high performance liquid chromatography methods. The ratio of oxidized and reduced forms of glutathione, as index of oxidative stress, was measured by liquid-chromatography tandem mass spectrometry method. NO synthase expression and activity were evaluated by immunofluorescence staining and ex-vivo experiments of L-[(15)N2]arginine conversion to L-[(15)N]citrulline respectively.
resultsIncreased amounts of asymmetric and symmetric dimethylarginines were found both in red blood cells and in plasma of CAD patients in respect to controls. Interestingly NO synthase expression and activity were reduced in CAD red blood cells. In contrast, oxidized/reduced glutathione ratio was increased in CAD and was associated to arginase activity.
conclusionOur study analyzed for the first time the whole metabolic pathway of L-arginine/NO, both in red blood cells and in plasma, highlighting an impairment of NO pathway in erythrocytes from CAD patients, associated with decreased NO synthase expression/activity and increased oxidative stress.
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