ArticleBiomicrofluidics2012
Biomarker-free dielectrophoretic sorting of differentiating myoblast multipotent progenitor cells and their membrane analysis by Raman spectroscopy.
Article in Biomicrofluidics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- Microfluidic-based electrically driven particle manipulation techniques for biomedical applications.RSC advances · 2025Review
- Extracellular vesicles may provide an alternative detoxification pathway during skeletal muscle myoblast ageing.Journal of extracellular biology · 2024Article
- Article
- Novel Sensing Technique for Stem Cells Differentiation Using Dielectric Spectroscopy of Their Proteins.Sensors (Basel, Switzerland) · 2023Article
- Article
- Lack of a skeletal muscle phenotype in adult human bone marrow stromal cells following xenogeneic-free expansion.Stem cell research & therapy · 2020Article
- New insights into anhydrobiosis using cellular dielectrophoresis-based characterization.Biomicrofluidics · 2019Article
- The Danger Signal Extracellular ATP Is Involved in the Immunomediated Damage of α-Sarcoglycan-Deficient Muscular Dystrophy.The American journal of pathology · 2019Article
- Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells.Stem cell reports · 2018Article
- It's Electric: When Technology Gives a Boost to Stem Cell Science.Current stem cell reports · 2018Article
- Separation of neural stem cells by whole cell membrane capacitance using dielectrophoresis.Methods (San Diego, Calif.) · 2018Review
- Size and dielectric properties of skeletal stem cells change critically after enrichment and expansion from human bone marrow: consequences for microfluidic cell sorting.Journal of the Royal Society, Interface · 2017Article
- Dynamic monitoring of single cell lysis in an impedance-based microfluidic device.Biomedical microdevices · 2016Article
- Increasing label-free stem cell sorting capacity to reach transplantation-scale throughput.Biomicrofluidics · 2014Article
- Membrane biophysics define neuron and astrocyte progenitors in the neural lineage.Stem cells (Dayton, Ohio) · 2014Article
- Dielectric characterization of costal cartilage chondrocytes.Biochimica et biophysica acta · 2014Article
- Hydrodynamic mechanisms of cell and particle trapping in microfluidics.Biomicrofluidics · 2013Review
- Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field.Analytical chemistry · 2013Review
- Differential electronic detector to monitor apoptosis using dielectrophoresis-induced translation of flowing cells (dielectrophoresis cytometry).Biomicrofluidics · 2013Article
- Efficient dielectrophoretic cell enrichment using a dielectrophoresis-well based system.Biomicrofluidics · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Myoblasts are muscle derived mesenchymal stem cell progenitors that have great potential for use in regenerative medicine, especially for cardiomyogenesis grafts and intracardiac cell transplantation. To utilise such cells for pre-clinical and clinical applications, and especially for personalized medicine, it is essential to generate a synchronised, homogenous, population of cells that display phenotypic and genotypic homogeneity within a population of cells. We demonstrate that the biomarker-free technique of dielectrophoresis (DEP) can be used to discriminate cells between stages of differentiation in the C2C12 myoblast multipotent mouse model. Terminally differentiated myotubes were separated from C2C12 myoblasts to better than 96% purity, a result validated by flow cytometry and Western blotting. To determine the extent to which cell membrane capacitance, rather than cell size, determined the DEP response of a cell, C2C12 myoblasts were co-cultured with GFP-expressing MRC-5 fibroblasts of comparable size distributions (mean diameter ∼10 μm). A DEP sorting efficiency greater than 98% was achieved for these two cell types, a result concluded to arise from the fibroblasts possessing a larger membrane capacitance than the myoblasts. It is currently assumed that differences in membrane capacitance primarily reflect differences in the extent of folding or surface features of the membrane. However, our finding by Raman spectroscopy that the fibroblast membranes contained a smaller proportion of saturated lipids than those of the myoblasts suggests that the membrane chemistry should also be taken into account.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.