ArticleProceedings of the National Academy of Sciences of the United States of America2013
Tuning a riboswitch response through structural extension of a pseudoknot.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Functional characterization of dynamic nascent RNA folding ensembles in real time.Science advances · 2026Article
- Two riboswitch classes that share a common ligand-binding fold show major differences in the ability to accommodate mutations.Nucleic acids research · 2024Article
- Mechanistic analysis of Riboswitch Ligand interactions provides insights into pharmacological control over gene expression.Nature communications · 2024Article
- Mechanistic Analysis of Riboswitch Ligand Interactions Provides Insights into Pharmacological Control over Gene Expression.bioRxiv : the preprint server for biology · 2024Article
- Structure-based investigations of the NAD+-II riboswitch.Nucleic acids research · 2023Article
- Unraveling the structure and biological functions of RNA triple helices.Wiley interdisciplinary reviews. RNA · 2020Review
- Nucleobase mutants of a bacterial preQThe Journal of biological chemistry · 2020Article
- Observation of preQRNA biology · 2019Article
- Article
- Practical synthesis ofMonatshefte fur chemie · 2019Article
- SHAPE probing pictures Mg2+-dependent folding of small self-cleaving ribozymes.Nucleic acids research · 2018Article
- Coupling Green Fluorescent Protein Expression with Chemical Modification to Probe Functionally Relevant Riboswitch Conformations in Live Bacteria.Biochemistry · 2018Article
- Life under the Microscope: Single-Molecule Fluorescence Highlights the RNA World.Chemical reviews · 2018Review
- Superior cellular activities of azido- over amino-functionalized ligands for engineered preQRNA biology · 2018Article
- Slow molecular recognition by RNA.RNA (New York, N.Y.) · 2017Article
- Pseudoknot Formation Seeds the Twister Ribozyme Cleavage Reaction Coordinate.Journal of the American Chemical Society · 2017Article
- Long-Range Interactions in Riboswitch Control of Gene Expression.Annual review of biophysics · 2017Review
- An Unconventional Acid-Labile Nucleobase Protection Concept for Guanosine Phosphoramidites in RNA Solid-Phase Synthesis.Chemistry (Weinheim an der Bergstrasse, Germany) · 2017Article
- Transcriptional pausing at the translation start site operates as a critical checkpoint for riboswitch regulation.Nature communications · 2017Article
- Molecular Buffers Permit Sensitivity Tuning and Inversion of Riboswitch Signals.ACS synthetic biology · 2016Article
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Authors and funding
6 authors.
Funding
Abstract
Structural and dynamic features of RNA folding landscapes represent critical aspects of RNA function in the cell and are particularly central to riboswitch-mediated control of gene expression. Here, using single-molecule fluorescence energy transfer imaging, we explore the folding dynamics of the preQ1 class II riboswitch, an upstream mRNA element that regulates downstream encoded modification enzymes of queuosine biosynthesis. For reasons that are not presently understood, the classical pseudoknot fold of this system harbors an extra stem-loop structure within its 3'-terminal region immediately upstream of the Shine-Dalgarno sequence that contributes to formation of the ligand-bound state. By imaging ligand-dependent preQ1 riboswitch folding from multiple structural perspectives, we reveal that the extra stem-loop strongly influences pseudoknot dynamics in a manner that decreases its propensity to spontaneously fold and increases its responsiveness to ligand binding. We conclude that the extra stem-loop sensitizes this RNA to broaden the dynamic range of the ON/OFF regulatory switch.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.