ArticleProceedings of the National Academy of Sciences of the United States of America2013
Attenuation of the posttranslational oscillator via transcription-translation feedback enhances circadian-phase shifts in Synechococcus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Intrinsic period stability of the cyanobacterial circadian oscillator across in vitro and in vivo conditions.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Effect of pH on the cyanobacterial circadian oscillator in vitro.Communications biology · 2025Article
- The Function, Regulation, and Mechanism of Protein Turnover in Circadian Systems inInternational journal of molecular sciences · 2024Review
- Perfecting the Life Clock: The Journey from PTO to TTFL.International journal of molecular sciences · 2023Review
- Orchestration of Circadian Timing by Macromolecular Protein Assemblies.Journal of molecular biology · 2020Review
- Damped circadian oscillation in the absence of KaiA in Synechococcus.Nature communications · 2020Article
- Structure, function, and mechanism of the core circadian clock in cyanobacteria.The Journal of biological chemistry · 2018Review
- Designing synthetic networks in silico: a generalised evolutionary algorithm approach.BMC systems biology · 2017Article
- Period Robustness and Entrainability of the Kai System to Changing Nucleotide Concentrations.Biophysical journal · 2017Article
- Timing the day: what makes bacterial clocks tick?Nature reviews. Microbiology · 2017Review
- Giving Time Purpose: The Synechococcus elongatus Clock in a Broader Network Context.Annual review of genetics · 2015Review
- Mixtures of opposing phosphorylations within hexamers precisely time feedback in the cyanobacterial circadian clock.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The cyanobacterial clock and metabolism.Current opinion in microbiology · 2014Review
- Hypersensitive photic responses and intact genome-wide transcriptional control without the KaiC phosphorylation cycle in the Synechococcus circadian system.Journal of bacteriology · 2014Article
- Metabolic compensation and circadian resilience in prokaryotic cyanobacteria.Annual review of biochemistry · 2014Review
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3 authors.
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Abstract
Circadian rhythms are endogenous biological timing processes that are ubiquitous in organisms ranging from cyanobacteria to humans. In the photoautotrophic unicellular cyanobacterium Synechococcus elongatus PCC 7942, under continuous light (LL) conditions, the transcription-translation feedback loop (TTFL) of KaiC generates a rhythmic change in the accumulation of KaiC relative to KaiA clock proteins (KaiC/KaiA ratio), which peak and trough at subjective dawn and dusk, respectively. However, the role of TTFL in the cyanobacterial circadian system remains unclear because it is not an essential requirement for the basic oscillation driven by the Kai-based posttranslational oscillator (PTO) and the transcriptional output mechanisms. Here, we show that TTFL is important for the circadian photic resetting property in Synechococcus. The robustness of PTO, which is exemplified by the amplitude of the KaiC phosphorylation cycle, changed depending on the KaiC/KaiA ratio, which was cyclic under LL. After cells were transferred from LL to the dark, the clock protein levels remained constant in the dark. When cells were transferred from LL to continuous dark at subjective dawn, the KaiC phosphorylation cycle was attenuated with a lower KaiC/KaiA ratio, a higher KaiC phosphorylation level, and a lower amplitude than that in cells transferred at subjective dusk. We also found that the greater the degree to which PTO was attenuated in continuous dark, the greater the phase shifts upon the subsequent light exposure. Based on these results, we propose that TTFL enhances resetting of the Kai-based PTO in Synechococcus.
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