ArticleProceedings of the National Academy of Sciences of the United States of America2013
Cryoelectron tomography reveals the sequential assembly of bacterial flagella in Borrelia burgdorferi.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
75 citing papers in PubMed, 116 citations in OpenAlex.
- Asymmetric architecture and adaptation of Treponema flagella.Nature communications · 2026Article
- Structural insights into the assembly and evolution of a complex bacterial flagellar motor.Nature microbiology · 2026Article
- The ancient E-ring in bacterial flagellar motors.FEMS microbiology reviews · 2026Review
- In Situ Structural Analysis of Leptospira spp. by Electron Cryotomography.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The structure of the complete extracellular bacterial flagellum reveals the mechanism of flagellin incorporation.Nature microbiology · 2025Article
- Article
- Inhibitors of Lysinoalanine Cross-Linking in the Flagella Hook as Antimicrobials against Spirochetes.ACS chemical biology · 2025Article
- Broadly conserved FlgV controls flagellar assembly and Borrelia burgdorferi dissemination in mice.Nature communications · 2024Article
- PilY1 regulates the dynamic architecture of the type IV pilus machine in Pseudomonas aeruginosa.Nature communications · 2024Article
- DnaA modulates the gene expression and morphology of the Lyme disease spirochete.bioRxiv : the preprint server for biology · 2024Article
- Article
- Broadly conserved FlgV controls flagellar assembly andbioRxiv : the preprint server for biology · 2024Article
- Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0 °C.eLife · 2023Article
- Artificial molecular machines: Design and observation.Smart molecules : open access · 2023Review
- Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0 °C.bioRxiv : the preprint server for biology · 2023Article
- An overview of the structure and function of the flagellar hook FlgE protein.World journal of microbiology & biotechnology · 2023Review
- Identification and Characterization of the Alternative σJournal of bacteriology · 2022Article
- Exploitation of a Bacterium-Encoded Lytic Transglycosylase by a Human Oral Lytic Phage To Facilitate Infection.Journal of virology · 2022Article
- Transcriptional and functional characterizations of multiple flagellin genes in spirochetes.Molecular microbiology · 2022Article
- The Current State of Knowledge on Baggio-Yoshinari Syndrome (Brazilian Lyme Disease-like Illness): Chronological Presentation of Historical and Scientific Events Observed over the Last 30 Years.Pathogens (Basel, Switzerland) · 2022Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
Abstract
Periplasmic flagella are essential for the distinctive morphology, motility, and infectious life cycle of the Lyme disease spirochete Borrelia burgdorferi. In this study, we genetically trapped intermediates in flagellar assembly and determined the 3D structures of the intermediates to 4-nm resolution by cryoelectron tomography. We provide structural evidence that secretion of rod substrates triggers remodeling of the central channel in the flagellar secretion apparatus from a closed to an open conformation. This open channel then serves as both a gateway and a template for flagellar rod assembly. The individual proteins assemble sequentially to form a modular rod. The hook cap initiates hook assembly on completion of the rod, and the filament cap facilitates filament assembly after formation of the mature hook. Cryoelectron tomography and mutational analysis thus combine synergistically to provide a unique structural blueprint of the assembly process of this intricate molecular machine in intact cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.