ArticleNucleic acids research2013
Metal ion as both a cofactor and a probe of metal-binding sites in a uranyl-specific DNAzyme: a uranyl photocleavage study.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 31 citations in OpenAlex.
- Solar-powered simultaneous highly efficient seawater desalination and highly specific target extraction with smart DNA hydrogels.Science advances · 2023Article
- Simultaneous FeScience advances · 2023Article
- DNA nano-pocket for ultra-selective uranyl extraction from seawater.Nature communications · 2020Article
- Colorimetric Detection of Uranyl Using a Litmus Test.Frontiers in chemistry · 2018Article
- Selective tumor cell death induced by irradiated riboflavin through recognizing DNA G-T mismatch.Nucleic acids research · 2017Article
- A microRNA-initiated DNAzyme motor operating in living cells.Nature communications · 2017Article
- A highly specific sodium aptamer probed by 2-aminopurine for robust Na+ sensing.Nucleic acids research · 2016Article
- Biochemical and Biophysical Understanding of Metal Ion Selectivity of DNAzymes.Inorganica chimica acta · 2016Article
- Fluorescent nanoprobes for sensing and imaging of metal ions: recent advances and future perspectives.Nano today · 2016Article
- Rational evolution of Cd2+-specific DNAzymes with phosphorothioate modified cleavage junction and Cd2+ sensing.Nucleic acids research · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
DNAzymes are known to bind metal ions specifically to carry out catalytic functions. Despite many studies since DNAzymes were discovered nearly two decades ago, the metal-binding sites in DNAzymes are not fully understood. Herein, we adopt uranyl photocleavage to probe specific uranyl-binding sites in the 39E DNAzyme with catalytically relevant concentrations of uranyl. The results indicate that uranyl binds between T23 and C25 in the bulge loop, G11 and T12 in the stem loop of the enzyme strand, as well as between T2.4 and G3 close to the cleavage site in the substrate strand. Control experiments using two 39E DNAzyme mutants revealed a different cleavage pattern of the mutated region. Another DNAzyme, the 8-17 DNAzyme, which has a similar secondary structure but shows no activity in the presence of uranyl, indicated a different uranyl-dependent photocleavage as well. In addition, a close correlation between the concentration-dependent photocleavage and enzymatic activities is also demonstrated. Together, these experiments suggest that uranyl photocleavage has been successfully used to probe catalytically relevant uranyl-binding sites in the 39E DNAzyme. As uranyl is the cofactor of the 39E DNAzyme as well as the probe, specific uranyl binding has now been identified without disruption of the structure.
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