ArticleMolecular plant pathology2014
Alternative sigma factor RpoN and its modulation protein YhbH are indispensable for Erwinia amylovora virulence.
Article in Molecular plant pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 57 citations in OpenAlex.
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- Virulence Regulation and Lifestyle Transitions: The Role of c-di-GMP and Two-Component Systems in Erwinia amylovora and Their Evolutionary Context Within Enterobacterales.Molecular plant pathology · 2026Review
- Analysis and comparison of the bacterial σ54 regulon: Evidence of phylogenetic trends in gene regulation.PloS one · 2025Article
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- Bacterial Stress Responses as Potential Targets in Overcoming Antibiotic Resistance.Microorganisms · 2022Review
- The Regulatory Functions of σInternational journal of molecular sciences · 2021Review
- Plant nitrate supply regulates Erwinia amylovora virulence gene expression in Arabidopsis.Molecular plant pathology · 2021Article
- The RsmA RNA-Binding Proteins inFrontiers in plant science · 2021Article
- Global Proteomic Profiling ofMicroorganisms · 2020Article
- The stringent response regulator (p) ppGpp mediates virulence gene expression and survival in Erwinia amylovora.BMC genomics · 2020Article
- EvaluatingPeerJ · 2020Article
- Phosphodiesterase Genes Regulate Amylovoran Production, Biofilm Formation, and Virulence in Erwinia amylovora.Applied and environmental microbiology · 2019Article
- Expression and regulation of the Pseudomonas aeruginosa hibernation promoting factor.Molecular microbiology · 2018Article
- Lon protease modulates virulence traits in Erwinia amylovora by direct monitoring of major regulators and indirectly through the Rcs and Gac-Csr regulatory systems.Molecular plant pathology · 2018Article
- Integration of multiple stimuli-sensing systems to regulate HrpS and type III secretion system in Erwinia amylovora.Molecular genetics and genomics : MGG · 2018Article
- Conservation of Erwinia amylovora pathogenicity-relevant genes among Erwinia genomes.Archives of microbiology · 2017Article
- The RNA-binding protein CsrA plays a central role in positively regulating virulence factors in Erwinia amylovora.Scientific reports · 2016Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In Erwinia amylovora, ECF (extracytoplasmic functions) alternative sigma factor HrpL regulates the transcription of hrp (hypersensitive response and pathogenicity)-type III secretion system (T3SS) genes by binding to a consensus sequence known as the hrp box in hrp gene promoters. In turn, the expression of hrpL has been proposed to be positively controlled by alternative sigma factor 54 (σ(54)) (RpoN) and HrpS, a member of the σ(54) enhancer-binding proteins (EBPs). However, the function of RpoN has not been characterized genetically in E. amylovora. In this study, we investigated the role of RpoN, a nitrogen limitation sigma factor, and its modulation protein YhbH, a novel ribosome-associated protein, in E. amylovora virulence. Our results showed that mutations in hrpS, hrpL, rpoN and yhbH, but not yfiA and rmf3, resulted in a nonpathogenic phenotype on immature pear fruits and apple shoots. Consistently, the expression of T3SS genes, including hrpL, dspE, hrpN and hrpA, was barely detected in hrpS, hrpL, rpoN and yhbH mutants. These mutants were also not capable of eliciting a hypersensitive response (HR) on tobacco; however, the overexpression of hrpL using an inducible promoter rescued the HR-eliciting abilities of these mutants. These results suggest that a sigma factor cascade exists in the regulatory networks of E. amylovora and regulates important virulence factors. On the basis of this study and previously reported data, a model is proposed for the regulation of T3SS in E. amylovora.
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