ArticleThe Plant journal : for cell and molecular biology2013
A 22-nt artificial microRNA mediates widespread RNA silencing in Arabidopsis.
Article in The Plant journal : for cell and molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 4 citations in OpenAlex.
- Acidic pH Condition Enhances Femtosecond Laser Photoinjection of Plasmid DNA into Intact Tobacco BY‑2.ACS omega · 2025Article
- Insights into the Red SeaweedPlants (Basel, Switzerland) · 2025Article
- Asymmetric bulges within hairpin RNA transgenes influence small RNA size, secondary siRNA production and viral defence.Nucleic acids research · 2024Article
- Multiple factors and features dictate the selective production of ct-siRNA in Arabidopsis.Communications biology · 2024Article
- Targeting theFrontiers in plant science · 2023Article
- Simple Webserver-Facilitated Method to Design and Synthesize Artificial miRNA Gene and Its Application in Engineering Viral Resistance.Plants (Basel, Switzerland) · 2022Article
- Systemic silencing of an endogenous plant gene by two classes of mobile 21-nucleotide artificial small RNAs.The Plant journal : for cell and molecular biology · 2022Article
- Transgene-free genome editing and RNAi ectopic application in fruit trees: Potential and limitations.Frontiers in plant science · 2022Review
- Resistance to Aflatoxin Accumulation in Maize Mediated by Host-Induced Silencing of theJournal of fungi (Basel, Switzerland) · 2021Article
- Topically delivered 22 nt siRNAs enhance RNAi silencing of endogenous genes in two species.Planta · 2021Article
- Exogenous RNA as a Regulatory Signal during a Plant's Interaction with the Biotic Environment: An Evolutionary Perspective and Future Applications in Agriculture.Plants (Basel, Switzerland) · 2021Article
- Systemic GFP silencing is associated with high transgene expression in Nicotiana benthamiana.PloS one · 2021Article
- Plin-amiR, a pre-microRNA-based technology for controlling herbivorous insect pests.Plant biotechnology journal · 2020Article
- Silencing of germacrene A synthase genes reduces guaianolide oxalate content inGM crops & food · 2020Article
- Genetically Modified Organism-Free RNA Interference: Exogenous Application of RNA Molecules in Plants.Plant physiology · 2020Article
- Barriers to Efficient Foliar Uptake of dsRNA and Molecular Barriers to dsRNA Activity in Plant Cells.Frontiers in plant science · 2020Article
- Molecular basis of transitivity in plant RNA silencing.Molecular biology reports · 2019Review
- Gene Regulation Mediated by microRNA-Triggered Secondary Small RNAs in Plants.Plants (Basel, Switzerland) · 2019Review
- Responsiveness and Adaptation to Salt Stress of the REDOX-RESPONSIVE TRANSCRIPTION FACTOR 1 (RRTF1) Gene are Controlled by its Promoter.Molecular biotechnology · 2019Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is known that 22-nucleotide (nt) microRNAs (miRNAs) derived from asymmetric duplexes trigger phased small-interfering RNA (phasiRNA) production from complementary targets. Here we investigate the efficacy of 22-nt artificial miRNA (amiRNA)-mediated RNA silencing relative to conventional hairpin RNA (hpRNA) and 21-nt amiRNA-mediated RNA silencing. CHALCONE SYNTHASE (CHS) was selected as a target in Arabidopsis thaliana due to the obvious and non-lethal loss of anthocyanin accumulation upon widespread RNA silencing. Over-expression of CHS in the pap1-D background facilitated visual detection of both local and systemic RNA silencing. RNA silencing was initiated in leaf tissues from hpRNA and amiRNA plant expression vectors under the control of an Arabidopsis RuBisCo small subunit 1A promoter (SSU). In this system, hpRNA expression triggered CHS silencing in most leaf tissues but not in roots or seed coats. Similarly, 21-nt amiRNA expression from symmetric miRNA/miRNA* duplexes triggered CHS silencing in all leaf tissues but not in roots or seed coats. However, 22-nt amiRNA expression from an asymmetric duplex triggered CHS silencing in all tissues, including roots and seed coats, in the majority of plant lines. This widespread CHS silencing required RNA-DEPENDENT RNA POLYMERASE6-mediated accumulation of phasiRNAs from the endogenous CHS transcript. These results demonstrate the efficacy of asymmetric 22-nt amiRNA-directed RNA silencing and associated phasiRNA production and activity, in mediating widespread RNA silencing of an endogenous target gene. Asymmetric 22-nt amiRNA-directed RNA silencing requires little modification of existing amiRNA technology and is expected to be effective in suppressing other genes and/or members of gene families.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.