ArticleJournal of proteome research2013
Affinity purification strategies for proteomic analysis of transcription factor complexes.
Article in Journal of proteome research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- MATR3 is an endogenous inhibitor of DUX4 in FSHD muscular dystrophy.Cell reports · 2023Article
- TMBIM5 is the CaEMBO reports · 2022Article
- Poly(ADP-ribosyl)ating pathway regulates development from stem cell niche to longevity control.Life science alliance · 2022Article
- PRISMA and BioID disclose a motifs-based interactome of the intrinsically disordered transcription factor C/EBPα.iScience · 2021Article
- Polymerase δ deficiency causes syndromic immunodeficiency with replicative stress.The Journal of clinical investigation · 2019Article
- Tandem Affinity Purification and Mass Spectrometry (TAP-MS) for the Analysis of Protein Complexes.Current protocols in protein science · 2019Article
- Interactome mapping defines BRG1, a component of the SWI/SNF chromatin remodeling complex, as a new partner of the transcriptional regulator CTCF.The Journal of biological chemistry · 2019Article
- Quantifying Tissue-Specific Overexpression of FOXO in Drosophila via mRNA Fluorescence In Situ Hybridization Using Branched DNA Probe Technology.Methods in molecular biology (Clifton, N.J.) · 2019Article
- MLL-fusion-driven leukemia requires SETD2 to safeguard genomic integrity.Nature communications · 2018Article
- Identification of Transcription Factor YY1 as a Regulator of a Prostate Cancer-Specific Pathway Using Proteomic Analysis.Journal of Cancer · 2017Article
- TNIP2 is a Hub Protein in the NF-κB Network with Both Protein and RNA Mediated Interactions.Molecular & cellular proteomics : MCP · 2016Article
- An Inducible Retroviral Expression System for Tandem Affinity Purification Mass-Spectrometry-Based Proteomics Identifies Mixed Lineage Kinase Domain-like Protein (MLKL) as an Heat Shock Protein 90 (HSP90) Client.Molecular & cellular proteomics : MCP · 2016Article
- Rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) for analysis of chromatin complexes.Nature protocols · 2016Article
- SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1.Nature · 2015Article
- Controlling for gene expression changes in transcription factor protein networks.Molecular & cellular proteomics : MCP · 2014Article
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Authors and funding
9 authors.
Funding
Abstract
Affinity purification (AP) coupled to mass spectrometry (MS) has been successful in elucidating protein molecular networks of mammalian cells. These approaches have dramatically increased the knowledge of the interconnectivity present among proteins and highlighted biological functions within different protein complexes. Despite significant technical improvements reached in the past years, it is still challenging to identify the interaction networks and the subsequent associated functions of nuclear proteins such as transcription factors (TFs). A straightforward and robust methodology is therefore required to obtain unbiased and reproducible interaction data. Here we present a new approach for TF AP-MS, exemplified with the CCAAT/enhancer binding protein alpha (C/EBPalpha). Utilizing the advantages of a double tag and three different MS strategies, we conducted a total of six independent AP-MS strategies to analyze the protein-protein interactions of C/EBPalpha. The resultant data were combined to produce a cohesive C/EBPalpha interactome. Our study describes a new methodology that robustly identifies specific molecular complexes associated with transcription factors. Moreover, it emphasizes the existence of TFs as protein complexes essential for cellular biological functions and not as single, static entities.
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