ArticlePLoS neglected tropical diseases2013
Polyethyleneimine mediated DNA transfection in schistosome parasites and regulation of the WNT signaling pathway by a dominant-negative SmMef2.
Article in PLoS neglected tropical diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Transgenic hookworm secretes anti-tetrodotoxin human single chain antibody.Nature communications · 2026Article
- Chemical modulation ofWellcome open research · 2023Article
- Transgenesis in parasitic helminths: a brief history and prospects for the future.Parasites & vectors · 2022Review
- Article
- Differential expression of microRNA between normally developed and underdeveloped female worms of Schistosoma japonicum.Veterinary research · 2020Article
- Heads or tails? Differential translational regulation in cercarial heads and tails of schistosome worms.PloS one · 2019Article
- Review
- The NIH-NIAID Schistosomiasis Resource Center at the Biomedical Research Institute: Molecular Redux.PLoS neglected tropical diseases · 2016Review
- Hsp70 May Be a Molecular Regulator of Schistosome Host Invasion.PLoS neglected tropical diseases · 2016Article
- Prospects and challenges of CRISPR/Cas genome editing for the study and control of neglected vector-borne nematode diseases.The FEBS journal · 2016Review
- MicroRNAs Are Involved in the Regulation of Ovary Development in the Pathogenic Blood Fluke Schistosoma japonicum.PLoS pathogens · 2016Article
- Cytometric analysis, genetic manipulation and antibiotic selection of the snail embryonic cell line Bge from Biomphalaria glabrata, the intermediate host of Schistosoma mansoni.International journal for parasitology · 2015Article
- Article
- Heterologous expression in Caenorhabditis elegans as an alternative approach to functional studies in Schistosoma mansoni.Frontiers in genetics · 2014Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Schistosomiasis is a serious global problem and the second most devastating parasitic disease following malaria. Parasitic worms of the genus Schistosoma are the causative agents of schistosomiasis and infect more than 240 million people worldwide. The paucity of molecular tools to manipulate schistosome gene expression has made an understanding of genetic pathways in these parasites difficult, increasing the challenge of identifying new potential drugs for treatment. Here, we describe the use of a formulation of polyethyleneimine (PEI) as an alternative to electroporation for the efficacious transfection of genetic material into schistosome parasites. We show efficient expression of genes from a heterologous CMV promoter and from the schistosome Sm23 promoter. Using the schistosome myocyte enhancer factor 2 (SmMef2), a transcriptional activator critical for myogenesis and other developmental pathways, we describe the development of a dominant-negative form of the schistosome Mef2. Using this mutant, we provide evidence that SmMef2 may regulate genes in the WNT pathway. We also show that SmMef2 regulates its own expression levels. These data demonstrate the use of PEI to facilitate effective transfection of nucleic acids into schistosomes, aiding in the study of schistosome gene expression and regulation, and development of genetic tools for the characterization of molecular pathways in these parasites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.